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Front Cell Dev Biol 2023 Jan 01;11:1260795. doi: 10.3389/fcell.2023.1260795.
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RNA localization during early development of the axolotl.

Šimková K , Naraine R , Vintr J , Soukup V , Šindelka R .

The asymmetric localization of biomolecules is critical for body plan development. One of the most popular model organisms for early embryogenesis studies is Xenopus laevis but there is a lack of information in other animal species. Here, we compared the early development of two amphibian species-the frog X. laevis and the axolotl Ambystoma mexicanum. This study aimed to identify asymmetrically localized RNAs along the animal-vegetal axis during the early development of A. mexicanum. For that purpose, we performed spatial transcriptome-wide analysis at low resolution, which revealed dynamic changes along the animal-vegetal axis classified into the following categories: profile alteration, de novo synthesis and degradation. Surprisingly, our results showed that many of the vegetally localized genes, which are important for germ cell development, are degraded during early development. Furthermore, we assessed the motif presence in UTRs of degraded mRNAs and revealed the enrichment of several motifs in RNAs of germ cell markers. Our results suggest novel reorganization of the transcriptome during embryogenesis of A. mexicanum to converge to the similar developmental pattern as the X. laevis.

PubMed ID: 37928901
PMC ID: PMC10620976
Article link: Front Cell Dev Biol

Species referenced: Xenopus laevis
Genes referenced: aco1 actb ap2b1 atp11b atp1a1 cap1 ccna1 crem csk dazl ddx5 dnd1 dvl3 dync1i2 fuca1 grip2 kif4a man2b1 mcm2 nanos1 nek9 pgc pi4ka pum2 rassf3 rbfox1 rbm24 rbpms2 rrp12 spire1 sulf1 tnf velo1 wee2 wnk2 wnt1 wnt5a wnt5b zfp36 zp4

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References [+] :
Abe, Minor zygotic gene activation is essential for mouse preimplantation development. 2018, Pubmed