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Nat Commun 2023 Oct 09;141:6316. doi: 10.1038/s41467-023-42049-0.
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A cyclin-dependent kinase-mediated phosphorylation switch of disordered protein condensation.

Valverde JM , Dubra G , Phillips M , Haider A , Elena-Real C , Fournet A , Alghoul E , Chahar D , Andrés-Sanchez N , Paloni M , Bernadó P , van Mierlo G , Vermeulen M , van den Toorn H , Heck AJR , Constantinou A , Barducci A , Ghosh K , Sibille N , Knipscheer P , Krasinska L , Fisher D , Altelaar M .

Cell cycle transitions result from global changes in protein phosphorylation states triggered by cyclin-dependent kinases (CDKs). To understand how this complexity produces an ordered and rapid cellular reorganisation, we generated a high-resolution map of changing phosphosites throughout unperturbed early cell cycles in single Xenopus embryos, derived the emergent principles through systems biology analysis, and tested them by biophysical modelling and biochemical experiments. We found that most dynamic phosphosites share two key characteristics: they occur on highly disordered proteins that localise to membraneless organelles, and are CDK targets. Furthermore, CDK-mediated multisite phosphorylation can switch homotypic interactions of such proteins between favourable and inhibitory modes for biomolecular condensate formation. These results provide insight into the molecular mechanisms and kinetics of mitotic cellular reorganisation.

PubMed ID: 37813838
PMC ID: PMC10562473
Article link: Nat Commun
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: cdk1 cdk2 cela2a cks1b dbf4 eif3a mapk1 mastl mcm4 mecp2 mtor ncl npm1 npy4r wee1
GO keywords: mitotic cell cycle [+]
Antibodies: GFP Ab29 H3f3a Ab26 Mecp2 Ab2 Ncl Ab3 Npm1 Ab2

Article Images: [+] show captions
References [+] :
Akdel, A structural biology community assessment of AlphaFold2 applications. 2022, Pubmed