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XB-ART-60181
Front Cell Dev Biol 2023 Jan 01;11:1168643. doi: 10.3389/fcell.2023.1168643.
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Recognition of H2AK119ub plays an important role in RSF1-regulated early Xenopus development.

Parast SM , Yu D , Chen C , Dickinson AJ , Chang C , Wang H .


Abstract
Polycomb group (PcG) proteins are key regulators of gene expression and developmental programs via covalent modification of histones, but the factors that interpret histone modification marks to regulate embryogenesis are less studied. We previously identified Remodeling and Spacing Factor 1 (RSF1) as a reader of histone H2A lysine 119 ubiquitination (H2AK119ub), the histone mark deposited by Polycomb Repressive Complex 1 (PRC1). In the current study, we used Xenopus laevis as a model to investigate how RSF1 affects early embryonic development and whether recognition of H2AK119ub is important for the function of RSF1. We showed that knockdown of Xenopus RSF1, rsf1, not only induced gastrulation defects as reported previously, but specific targeted knockdown in prospective neural precursors induced neural and neural crest defects, with reductions of marker genes. In addition, similar to knockdown of PRC1 components in Xenopus, the anterior-posterior neural patterning was affected in rsf1 knockdown embryos. Binding of H2AK119ub appeared to be crucial for rsf1 function, as a construct with deletion of the UAB domain, which is required for RSF1 to recognize the H2AK119ub nucleosomes, failed to rescue rsf1 morphant embryos and was less effective in interfering with early Xenopus development when ectopically expressed. Furthermore, ectopic deposition of H2AK119ub on the Smad2 target gene gsc using a ring1a-smad2 fusion protein led to ectopic recruitment of RSF1. The fusion protein was inefficient in inducing mesodermal markers in the animal region or a secondary axis when expressed in the ventral tissues. Taken together, our results reveal that rsf1 modulates similar developmental processes in early Xenopus embryos as components of PRC1 do, and that RSF1 acts at least partially through binding to the H2AK119ub mark via the UAB domain during development.

PubMed ID: 37529237
PMC ID: PMC10389277
Article link: Front Cell Dev Biol
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: chrd.1 egr2 gsc h2ac21 hoxd10 hoxd4 hoxd9 nodal1 pou5f3.2 prc1 ring1 rnf2 rsf1 smad2 sox10 sox9 tbxt
GO keywords: gastrulation [+]
Antibodies: GFP Ab7 HIST1H2AA Ab1 actb Ab11 rsf1 Ab1
Morpholinos: rsf1 MO1


Article Images: [+] show captions
References [+] :
Baile, Roles of Polycomb complexes in regulating gene expression and chromatin structure in plants. 2022, Pubmed