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Proc Natl Acad Sci U S A 2017 Sep 19;11438:E7949-E7958. doi: 10.1073/pnas.1711158114.
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Role of remodeling and spacing factor 1 in histone H2A ubiquitination-mediated gene silencing.

Zhang Z , Jones AE , Wu W , Kim J , Kang Y , Bi X , Gu Y , Popov IK , Renfrow MB , Vassylyeva MN , Vassylyev DG , Giles KE , Chen D , Kumar A , Fan Y , Tong Y , Liu CF , An W , Chang C , Luo J , Chow LT , Wang H .

Posttranslational histone modifications play important roles in regulating chromatin-based nuclear processes. Histone H2AK119 ubiquitination (H2Aub) is a prevalent modification and has been primarily linked to gene silencing. However, the underlying mechanism remains largely obscure. Here we report the identification of RSF1 (remodeling and spacing factor 1), a subunit of the RSF complex, as a H2Aub binding protein, which mediates the gene-silencing function of this histone modification. RSF1 associates specifically with H2Aub, but not H2Bub nucleosomes, through a previously uncharacterized and obligatory region designated as ubiquitinated H2A binding domain. In human and mouse cells, genes regulated by RSF1 overlap significantly with those controlled by RNF2/Ring1B, the subunit of Polycomb repressive complex 1 (PRC1) which catalyzes the ubiquitination of H2AK119. About 82% of H2Aub-enriched genes, including the classic PRC1 target Hox genes, are bound by RSF1 around their transcription start sites. Depletion of H2Aub levels by Ring1B knockout results in a significant reduction of RSF1 binding. In contrast, RSF1 knockout does not affect RNF2/Ring1B or H2Aub levels but leads to derepression of H2Aub target genes, accompanied by changes in H2Aub chromatin organization and release of linker histone H1. The action of RSF1 in H2Aub-mediated gene silencing is further demonstrated by chromatin-based in vitro transcription. Finally, RSF1 and Ring1 act cooperatively to regulate mesodermal cell specification and gastrulation during Xenopus early embryonic development. Taken together, these data identify RSF1 as a H2Aub reader that contributes to H2Aub-mediated gene silencing by maintaining a stable nucleosome pattern at promoter regions.

PubMed ID: 28855339
PMC ID: PMC5617306
Article link: Proc Natl Acad Sci U S A
Grant support: [+]

Species referenced: Xenopus
Genes referenced: anxa6 bclaf1 bmf chd1 chrd dkk1 eftud2 f10 fbxo2 gapdh grem1 h2ac21 h4c4 hoxb7 hoxb8 hoxc6 ilf3 kcnma1 klf2 lgals4.2 lmna noc3l nop58 prc1 rbmx ring1 rnf2 rpl23a rpl9 rps4x rsf1 sf3b2 snw1 socs1 spen ssrp1 tbxt top1 usp16 wnt8a
GO keywords: mesodermal cell fate specification [+]
Antibodies: H3f3a Ab33 HIST1H2AA Ab2
Morpholinos: ring1 MO1 rnf2 MO1 rsf1 MO1

Article Images: [+] show captions
References [+] :
An, Selective requirements for histone H3 and H4 N termini in p300-dependent transcriptional activation from chromatin. 2002, Pubmed, Xenbase