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Elife 2022 Dec 13;11. doi: 10.7554/eLife.84694.
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Cell cycle-specific loading of condensin I is regulated by the N-terminal tail of its kleisin subunit.

Tane S , Shintomi K , Kinoshita K , Tsubota Y , Yoshida MM , Nishiyama T , Hirano T .

Condensin I is a pentameric protein complex that plays an essential role in mitotic chromosome assembly in eukaryotic cells. Although it has been shown that condensin I loading is mitosis specific, it remains poorly understood how the robust cell cycle regulation of condensin I is achieved. Here, we set up a panel of in vitro assays to demonstrate that cell cycle-specific loading of condensin I is regulated by the N-terminal tail (N-tail) of its kleisin subunit CAP-H. Deletion of the N-tail accelerates condensin I loading and chromosome assembly in Xenopus egg mitotic extracts. Phosphorylation-deficient and phosphorylation-mimetic mutations in the CAP-H N-tail decelerate and accelerate condensin I loading, respectively. Remarkably, deletion of the N-tail enables condensin I to assemble mitotic chromosome-like structures even in interphase extracts. Together with other extract-free functional assays in vitro, our results uncover one of the multilayered mechanisms that ensure cell cycle-specific loading of condensin I onto chromosomes.

PubMed ID: 36511239
PMC ID: PMC9797191
Article link: Elife

Species referenced: Xenopus laevis
Genes referenced: pnp smc2 smc4
GO keywords: condensin complex [+]
Antibodies: Ncapd2 Ab2 Ncapd3 Ab2 Ncapg Ab1 Ncaph Ab1 Smc2 Ab1 Smc2 Ab2 Smc4 Ab1 Top2a Ab1

Article Images: [+] show captions
References [+] :
Batty, Mitotic Chromosome Mechanics: How Cells Segregate Their Genome. 2019, Pubmed