XB-ART-59268
Elife
2022 Aug 19;11. doi: 10.7554/eLife.78984.
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Molecular dissection of condensin II-mediated chromosome assembly using in vitro assays.
Yoshida MM
,
Kinoshita K
,
Aizawa Y
,
Tane S
,
Yamashita D
,
Shintomi K
,
Hirano T
.
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In vertebrates, condensin I and condensin II cooperate to assemble rod-shaped chromosomes during mitosis. Although the mechanism of action and regulation of condensin I have been studied extensively, our corresponding knowledge of condensin II remains very limited. By introducing recombinant condensin II complexes into Xenopus egg extracts, we dissect the roles of its individual subunits in chromosome assembly. We find that one of two HEAT subunits, CAP-D3, plays a crucial role in condensin II-mediated assembly of chromosome axes, whereas the other HEAT subunit, CAP-G2, has a very strong negative impact on this process. The structural maintenance of chromosomes ATPase and the basic amino acid clusters of the kleisin subunit CAP-H2 are essential for this process. Deletion of the C-terminal tail of CAP-D3 increases the ability of condensin II to assemble chromosomes and further exposes a hidden function of CAP-G2 in the lateral compaction of chromosomes. Taken together, our results uncover a multilayered regulatory mechanism unique to condensin II, and provide profound implications for the evolution of condensin II.
???displayArticle.pubmedLink??? 35983835
???displayArticle.pmcLink??? PMC9433093
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???displayArticle.grants??? [+]
#18H05276 Japan Society for the Promotion of Science , #20H0593 Japan Society for the Promotion of Science , #20K15723 Japan Society for the Promotion of Science , #15K06959 Japan Society for the Promotion of Science , #19K06499 Japan Society for the Promotion of Science , #18H02381 Japan Society for the Promotion of Science , #19H05755 Japan Society for the Promotion of Science
Species referenced: Xenopus laevis
Genes referenced: cdk1 npm1
GO keywords: mitotic cell cycle [+]
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Figure 1. Recombinant condensin II holocomplex can functionally replace endogenous condensin II in Xenopus egg extracts. (A) A schematic diagram of the condensin II holocomplex that is composed of two structural maintenance of chromosomes (SMC) subunits (SMC2 and SMC4), two HEAT subunits (CAP-D3 and CAP-G2), and a kleisin subunit (CAP-H2). (B) Mouse sperm nuclei were incubated with Δmock, Δcond I, or Δcond I/II extracts that had been supplemented with a control buffer (buffer) or recombinant (rec) condensin II holocomplex, holo(WT), at a final concentration of 200 nM. After 150 min, reaction mixtures were fixed, split, and labeled with an antibody against either Xenopus CAP-H2 (XCAP-H2) or human CAP-H2 (hCAP-H2). DNA was counterstained with DAPI (4′,6-diamidino-2-phenylindole). Shown here is a representative image from over 15 chromosome clusters examined per condition. Scale bar, 10 µm. (C) A schematic representation of the chromosome structures observed in Δmock, Δcond I, or Δcond I/II extracts. Rod-shaped chromosomes and chenille-like chromosomes are assembled in Δmock extract and Δcond I extract, respectively. Δcond I/II extract produces an amorphous mass of chromatin (‘clouds’). The addition of recombinant condensin II (rec cond II) to the same extract restores its ability to assemble chenille-like chromosomes. (D) A single chromosome assembled in Δcond I extract with buffer and Δcond I/II extract with 200 nM holo(WT) from samples from experiment (B) in comparison to a chenille stem. Scale bars for chromosomes, 2 µm. (E) DAPI intensities from the experiment described in (B) were measured along lines drawn perpendicular to chromosome axes to create line scan profiles for the width of chromosomes assembled in the Δcond I extract with buffer and Δcond I/II extract with 200 nM holo(WT) (n=20 chromosomes). The mean and SD were normalized individually to the DAPI intensity (arbitrary unit [a.u.]) at the center of chromosome axes (distance = 0 μm) within each set. A dataset from a single representative experiment out of three repeats is shown. | |
Figure 1—figure supplement 1. Recombinant condensin II complexes used in the study. (A) Recombinant condensin II holocomplex (holo[WT]), ΔG2(WT), ΔD3(WT), and ΔD3ΔG2(WT) were purified and subjected to SDS-PAGE. The gel was stained with Coomassie Brilliant Blue (CBB). (B) Recombinant condensin II holocomplexes and ΔG2 subcomplexes containing either wild-type (WT) or transition state (TR) mutant version of the structural maintenance of chromosomes (SMC) subunits. (C) Recombinant condensin II holocomplexes and ΔG2 subcomplexes containing either WT or C-tail truncated version (D3–dC) of the CAP-D3 subunit. (D) Recombinant condensin II holocomplexes containing either WT, a single set of basic cluster mutations (H2-BC1D or H2-BC2D), or both sets of basic cluster mutations (H2-BC1/2D) of the CAP-H2 subunit. (E) Recombinant condensin II holo(WT) was incubated with or without λ protein phosphatase (PPase) for 60 min and subjected to immunoblotting. hCAP-H2 migrated as two bands without phosphatase treatment but migrated as a single band after protein phosphatase treatment, indicating that a fraction of hCAP-H2 is phosphorylated when expressed in insect cells. | |
Figure 2. Chromosome association of condensin II is decreased by CAP-D3 deletion but is increased by CAP-G2 deletion. (A) (Left) Mouse sperm nuclei were incubated with Δcond I/II extracts that had been supplemented with control buffer (buffer), recombinant condensin II holocomplex (holo[WT]) or its subcomplexes (ΔG2[WT], ΔD3[WT], and ΔD3ΔG2[WT]) at 200 nM. After 150 min, reaction mixtures were fixed, and labeled with anti-hCAP-H2 antibody. DNA was counterstained with DAPI. The images of the hCAP-H2 signal were captured at different relative exposure times (1 × and 1/4 ×) to show non-saturated signal intensities of chromosome structures produced by ΔG2(WT). Shown here is a representative image from over 15 chromosome clusters examined per condition. Scale bar, 10 µm. (Right) Schematic diagrams of the holocomplex and subcomplexes. (B) Signal intensities of hCAP-H2 from the experiment described in (A) were divided by DAPI signal intensities and the mean values were normalized relative to the value by holo(WT). The mean ± SD is shown (n=15 chromosome clusters). The p-values listed were assessed by two-tailed Welch’s t-test. (C) Mouse sperm nuclei were incubated with Δcond I/II extracts that had been supplemented with holo(WT) or ΔG2(WT) at concentrations indicated. After 150 min, reaction mixtures were fixed and processed for immunofluorescence as in (A). Shown here is a representative image from over 15 chromosome clusters examined per condition. The right panels show cropped images of the orange sections of the left panels. Scale bar, 10 µm (left) and 5 µm (right). (D) DAPI and hCAP-H2 intensities from the experiment described in (C) were measured along lines drawn perpendicular to the chromosome to create line scan profiles for the width of chromosomes assembled in Δcond I/II extract with 200 nM holo(WT) or either 25 nM or 200 nM ΔG2(WT) (n=20 chromosomes). The mean and standard deviation were normalized individually to the DAPI intensities (arbitrary unit [a.u.]) at the center of chromosome axes (distance = 0 μm) within each set. Intensities of hCAP-H2 were normalized relative to the central value by 200 nM holo(WT) addition. A dataset from a single representative experiment out of three repeats is shown. WT: wild-type. | |
Figure 2—figure supplement 1. Immunodepletion of endogenous condensin complexes and basic characterization of recombinant condensin II complexes in Xenopus egg extracts. (A) Xenopus egg extracts were immunodepleted with control IgG (Δmock), antibodies against endogenous condensin I subunit (Δcond I extract), or antibodies against both endogenous condensin I and II subunits (Δcond I/II extract). An aliquot of each immunodepleted extract was analyzed by immunoblotting with antibodies to estimate the efficiency of immunodepletion with decreasing amounts of Δmock extract (100, 50, 20, and 5%). Xenopus topoisomerase IIα (Xtopo IIα) acts as a loading control. Asterisks (*) indicate non-specific bands. Immunodepletion of Xenopus CAP-H2 (XCAP-H2) was evaluated with two different affinity-purified antibodies (AfR201 and AfR202) raised against the same antigen to confirm that the band migrating slightly slower than XCAP-H2 is a non-specific band cross-reacted only with AfR201 but not with AfR202. (B) Mouse sperm nuclei were incubated in Δcond I/II extracts supplemented with control buffer (buffer) or holo(WT) at increasing concentrations from 50 nM to 300 nM. After 150 min, reaction mixtures were fixed, labeled with anti-hCAP-H2 antibody, and stained by DAPI. Shown here is a representative image from over 15 chromosome clusters examined per condition. The change in chromosomal morphology from cloud to chenille-like chromosomes by recombinant mammalian condensin II holo(WT) is concentration-dependent. Scale bar, 10 µm. (C) Mouse sperm nuclei were incubated in Δcond I/II extracts supplemented with control buffer (buffer), holo(WT) at 200 nM or ΔG2(WT) at 200 nM. Reaction mixtures were fixed at 0, 15, 30, 60, 90, 120, and 150 min, and labeled with anti-hCAP-H2 antibody. DNA was counterstained by DAPI. Shown here is a representative image from over 15 chromosome clusters examined per condition. ΔG2(WT) accumulates on chromatin and induces its structural changes at much earlier time points than holo(WT). Scale bar, 10 µm. WT: wild-type. | |
Figure 3. The structural maintenance of chromosome (SMC) ATPase cycle is essential for condensin II function. (A) Mouse sperm nuclei were incubated with Δcond I/II extracts that had been supplemented with condensin II holo(WT) and holo(SMC-TR) at 200 nM, or ΔG2(WT) and ΔG2(SMC-TR) at either 200 nM or 25 nM. After 150 min, reaction mixtures were fixed and labeled with anti-hCAP-H2 antibody. DNA was counterstained with DAPI. Shown here is a representative image from over 15 chromosome clusters examined per condition. The images of hCAP-H2 signal were first captured at a single exposure time (1/3 ×) and then increased digitally (1 ×) to visualize signals from chromosomes of complexes apart from ΔG2(WT) at 200 nM. Scale bar, 10 µm. (B) Signal intensities of hCAP-H2 from the experiment described in (A) were divided by DAPI intensities and the mean values were normalized relative to the value by holo(WT). The mean ± SD is shown (n=15 chromosome clusters). The p-values listed were assessed by two-tailed Welch’s t-test. A dataset from a single representative experiment out of two repeats is shown. (C) ATPase rates of recombinant condensin II holo(WT), ΔG2(WT), holo(SMC-TR), and ΔG2(SMC-TR) in the presence or absence of double-stranded DNA (dsDNA). The error bar represents the SD from three performed repeats. P values listed were assessed by two-tailed t-test. WT: wild-type. | |
Figure 4. The CAP-D3 C-tail negatively regulates condensin II-mediated chromosome assembly. (A) A schematic diagram of the full-length hCAP-D3 (D3–FL) and hCAP-D3 lacking the C-terminal tail (D3–dC). (B) Mouse sperm nuclei were incubated in Δcond I/II extracts that had been supplemented with condensin II holo(WT) or holo(D3-dC) at either 25, 50, or 200 nM. After 150 min, reaction mixtures were fixed and labeled with anti-hCAP-H2 antibody. DNA was counterstained with DAPI. Shown here is a representative image from over 15 chromosome clusters examined per condition. Scale bar, 10 µm. (C) DAPI and hCAP-H2 intensities from the experiment described in (B) were measured along lines drawn perpendicular to chromosome axes to create line scan profiles for the width of chromosomes assembled in Δcond I/II extract with 200 nM holo(WT) or either 50 nM or 200 nM holo(D3-dC) (n=20 chromosomes). The mean and SD were normalized individually to the DAPI intensities (arbitrary unit [a.u.]) at the center of chromosome axes (distance = 0 μm) within each set. Intensities of hCAP-H2 were normalized relative to the central value by 200 nM holo(WT) addition. A dataset from a single representative experiment out of three repeats is shown. (D) Mouse sperm nuclei were incubated in Δcond I/II extracts that had been supplemented with condensin II ΔG2(WT) or ΔG2(D3-dC) at either 25, 50, or 200 nM in the same experiment as (B). After 150 min, reaction mixtures were fixed and labeled with anti-hCAP-H2 antibody. DNA was counterstained with DAPI. Shown here is a representative image from over 15 chromosome clusters examined per condition. Scale bar, 10 µm. WT: wild-type. | |
Figure 4—figure supplement 1. Additional characterization of deletion of the CAP-D3 C-tail. (A) Sequence alignment of the C-tail of the CAP-D3 orthologs from Danio rerio (DrCAP-D3), Xenopus laevis (XCAP-D3), Gallus (GgCAP-D3), Mus musculus (mCAP-D3) and human (hCAP-D3). Note that hCAP-D3-dC lacks the C-tail containing multiple potential CDK1 phosphorylation consensus sites (red), which include T1415 and S1474 (blue). (B) Xenopus egg extracts were immunodepleted with antibodies against both endogenous condensin I and II subunits (Δcond I/II extract). The extracts were supplemented with control buffer (buffer), holo(WT), or holo(D3-dC) without sperm nuclei. Aliquots were taken at the indicated time points and subjected to immunoblotting with the indicated antibodies, including anti-phospho-specific antibodies against hCAP-D3 pT1415 and pS1474. Xenopus topoisomerase IIα (Xtopo IIα) was used as a loading control. Asterisks (*) indicate non-specific bands. Note that full-length hCAP-D3 (D3–FL) migrated slower as incubation time increased, which is accompanied by phosphorylation at T1415 and S1474. In contrast, CAP-D3 lacking the C-tail (D3–dC) did not change in its mobility upon incubation. (C) ATPase rate of holo(D3-dC) was compared with that of holo(WT) and other mutant complexes in the absence or presence of double-stranded DNA (dsDNA). The error bar represents the SD from three repeats. The p-values listed were assessed by two-tailed t-test. Note that part of the data derived from the same set of experiments was shown in Figure 3C. | |
Figure 4—figure supplement 2. Additional profiles of chromosomes assembled by holo(WT), holo(D3-dC), ΔG2(WT), and ΔG2(D3-dC) from the experiment described in Figure 4. (A) Chromosomes assembled by holo(WT), holo(D3-dC), and holo(D3-dC) added at the concentrations indicated. Shown here are blowups of part of the images presented in Figure 4B. Scale bar, 5 µm. (B) to (D) Chromosomes were assembled in Δcond I/II extract that had been supplemented with holo(WT), holo(D3-dC), ΔG2(WT), or ΔG2(D3-dC) at three different concentrations as described in Figure 4B and D. DAPI and hCAP-H2 intensities were measured along lines drawn perpendicular to chromosome axes to create line scan profiles for the width of chromosomes. The dataset shown is from the same single representative experiment as Figure 4C out of three repeats (n=20 chromosomes). The mean and SD were normalized relative to the DAPI and hCAP-H2 intensities (arbitrary unit [a.u.]) at the center of chromosome axes (distance = 0 μm) formed by 200 nM holo(WT) addition. (B) Profiles of hCAP-H2 intensities for ΔG2(WT) and ΔG2(D3-dC) added at concentrations of 25 nM and 50 nM were compared. (C) Profiles of DAPI intensities for ΔG2(WT), ΔG2(D3-dC), and holo(D3-dC) added at different concentrations were compared to the profile for holo(WT) added at 200 nM. Note that DAPI intensities at the axial region decreased in a dose-dependent manner in the cases of ΔG2(WT) and ΔG2(D3-dC) addition, whereas they increase in the case of holo(D3-dC) addition. (D) Profiles of DAPI and hCAP-H2 intensities for holo(WT), holo(D3-dC), and ΔG2(WT) added at 200 nM were compared. WT: wild-type. | |
Figure 4—figure supplement 3. Deletion of the CAP-D2 C-tail in condensin I has little impact on chromosome assembly in Xenopus egg extracts. (A) A schematic diagram of the full-length hCAP-D2 (D2–FL) and hCAP-D2 lacking the C-terminal tail (D2–dC). (B) Recombinant condensin I holocomplexes containing either wild-type (WT) or C-tail truncated (D2–dC) version of hCAP-D2 were purified and subjected to SDS-PAGE. The gel was stained with Coomassie Brilliant Blue. (C) Mouse sperm nuclei were incubated in Δcond I/II extracts that had been supplemented with recombinant condensin I holo(WT) or holo(D2-dC) at 35 nM. After 150 min, reaction mixtures were fixed and labeled with anti-mSMC4 antibody. DNA was counterstained with DAPI. Shown here is a representative image from over 15 chromosome clusters examined per condition. Scale bar, 10 µm. (D) DAPI and mSMC4 intensities from the experiment described in (C) were measured along lines drawn perpendicular to chromosome axes to create line scan profiles for the width of chromosomes assembled in Δcond I/II extract with either recombinant condensin I holo(WT) or holo(D2-dC) at 35 nM (n=20 chromosomes). The mean and SD were normalized individually to the DAPI intensities (arbitrary unit [a.u.]) at the center of chromosome axes (distance = 0 μm) within each set. Intensities of mSMC4 were normalized relative to the central value by 35 nM condensin I holo(WT) addition. Deletion of the CAP-D2 C-tail showed little effect on the accumulation of condensin I on the axis and on the rod-shaped chromosome morphology compared to those by holo(WT). SMC, structural maintenance of chromosomes; WT: wild-type. | |
Figure 5. The basic amino acid clusters of CAP-H2 synergistically contribute to condensin II functions. (A) A schematic diagram of domain organization of hCAP-H2. CAP-H2 has five motifs that are well conserved among vertebrate species (motifs I to V). Shown here is a sequence alignment of the CAP-H2 orthologs from Danio rerio (DrCAP-H2), Xenopus laevis (XCAP-H2), Gallus gallus (GgCAP-H2), Mus musculus (mCAP-H2) and human (hCAP-H2). Conserved residues are labeled in dark blue (Y/F/W), light blue (P) and gray (I/M/L*). Within the central region, two basic amino acid clusters (BC1 and BC2 shown in red). The BC1D and BC2D mutations were created by substituting all basic residues (K/R) conserved in BC1 and BC2, respectively, with aspartic acid residues (D). The BC1/2D mutations are a combination of the BC1D and BC2D mutations. (B) Mouse sperm nuclei were incubated with Δcond I/II extracts that had been supplemented with holo(WT), holo(H2-BC1D), holo(H2-BC2D) and holo(H2-BC1/2D) at 200 nM. After 150 min, reaction mixtures were fixed and labeled with anti-hCAP-H2 antibody. DNA was counterstained with DAPI. Shown here is a representative image from over 15 chromosome clusters examined per condition. Scale bar, 10 µm. (C) (Left) Signal intensities of hCAP-H2 from the experiment described in (B) were divided by DAPI intensities and the mean values were normalized relative to the values by holo(WT). (Right) Signal intensities of hCAP-H2 accumulated at the axes were divided by the overall hCAP-H2 intensities on the chromosomal DNA and the mean values were normalized to the value by holo(WT). The mean ± SD is shown (n=15 chromosome clusters). The p-values listed were assessed by two-tailed Welch’s t-test. A dataset from a single representative experiment out of two repeats is shown. | |
Figure 5—figure supplement 1. Deletion of CAP-G2 partially rescues mutations in the basic amino acid clusters of CAP-H2. (A) Recombinant mammalian condensin II holocomplex (holo) and ΔG2 subcomplex harboring either wild-type (WT) or mutations in the H2 basic amino acid cluster (H2-BC1D, H2-BC2D, and H2-BC1/2D) were purified and subjected to SDS-PAGE. The gel was stained with Coomassie Brilliant Blue. (B) Mouse sperm nuclei were incubated in Δcond I/II extracts that had been supplemented with condensin II holocomplex (WT, H2-BC1D, H2-BC2D, or H2-BC1/2D) at 200 nM or ΔG2 subcomplexes (WT, H2-BC1D, H2-BC2D, and H2-BC1/2D) at either 25 or 200 nM. After incubation for 150 min, reactions were fixed and labeled with anti-hCAP-H2 antibody. DNA was counterstained with DAPI. Shown here is a representative image from over 15 chromosome clusters examined per condition. Scale bar, 10 µm. In samples with 200 nM addition, ΔG2 subcomplexes were overloaded on chromosomal axes but only ΔG2(WT) made longer, continuous axes while ΔG2 with H2 basic cluster mutations. | |
Figure 6. ATP-stimulated chromatin binding of condensin II can be recapitulated in an extract-free assay. (A) Xenopus sperm nuclei were pre-incubated with a buffer containing recombinant nucleoplasmin (Npm2) in the absence or presence of ATP for 30 min, and then holo(WT), ΔG2(WT), ΔD3(WT), ΔD3ΔG2(WT), or holo(TR) was added at 50 nM. After 120 min, reaction mixtures were fixed and labeled with anti-mSMC4 antibody. DNA was counterstained with DAPI. Shown here is a representative image from over 20 nuclei examined per condition. Scale bar, 10 µm. (B) (Left) Signal intensities of hCAP-H2 were divided by DAPI intensities and the mean values were normalized relative to the values by holo(WT) without ATP addition (-ATP). (Right) Areas of DAPI signals were measured and the mean values were normalized to values by holo(WT) without ATP addition (-ATP). The mean ± SD is shown (n=20 sperm nuclei). The p-values listed were assessed by two-tailed Welch’s t-test. SMC, structural maintenance of chromosomes; WT: wild-type. | |
Figure 6—figure supplement 1. Characterization of holo(D3-dC) in an extract-free chromatin binding assay. (A) Xenopus sperm nuclei were pre-incubated with a buffer containing recombinant nucleoplasmin (Npm2) in the absence or presence of ATP for 30 min, and then holo(WT) or holo(D3-dC) was added at 50 nM. After 120 min, reaction mixtures were fixed and labeled with anti-mSMC4 antibody. DNA was counterstained with DAPI. Shown here is a representative image from over 20 nuclei examined per condition. Scale bar, 10 µm. (B) (Left) Signal intensities of hCAP-H2 from the experiment described in (A) were divided by DAPI intensities and the mean values were normalized to values by holo(WT) without ATP addition (-ATP). (Right) Areas of DAPI signals were measured and the mean values were normalized relative to the values by holo(WT) without ATP addition (-ATP). The mean ± SD is shown (n=20 sperm nuclei). P values listed were assessed by two-tailed Welch’s t-test. (C) Xenopus sperm nuclei were pre-incubated with a buffer containing recombinant nucleoplasmin (Npm2) in the absence or presence of ATP for 30 min, and then holo(WT) or a holocomplex harboring either H2-BC1D, H2-BC2D, or H2-BC1/2D mutations was added at 50 nM. After 120 min, reaction mixtures were fixed and labeled with anti-mSMC4 antibody. DNA was counterstained with DAPI. Shown here is a representative image from over 20 nuclei examined per condition. Scale bar, 10 µm. (D) (Left) Signal intensities of hCAP-H2 from the experiment described in (C) were divided by DAPI intensities and the mean values were normalized to the values by holo(WT) without ATP addition (-ATP). (Right) Areas of DAPI signals were measured and the mean values were normalized relative to the values by holo(WT) without ATP addition (-ATP). The mean ± SD is shown (n=20 sperm nuclei). P values listed were assessed by two-tailed Welch’s t-test. SMC, structural maintenance of chromosomes; WT: wild-type. |
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