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PLoS One 2018 Jan 18;131:e0191751. doi: 10.1371/journal.pone.0191751.
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Gene expression of the two developmentally regulated dermatan sulfate epimerases in the Xenopus embryo.

Gouignard N , Schön T , Holmgren C , Strate I , Taşöz E , Wetzel F , Maccarana M , Pera EM .

Chondroitin sulfate (CS)/dermatan sulfate (DS) proteoglycans are abundant on the cell surface and in the extracellular matrix and have important functions in matrix structure, cell-matrix interaction and signaling. The DS epimerases 1 and 2, encoded by Dse and Dsel, respectively, convert CS to a CS/DS hybrid chain, which is structurally and conformationally richer than CS, favouring interaction with matrix proteins and growth factors. We recently showed that Xenopus Dse is essential for the migration of neural crest cells by allowing cell surface CS/DS proteoglycans to adhere to fibronectin. Here we investigate the expression of Dse and Dsel in Xenopus embryos. We show that both genes are maternally expressed and exhibit partially overlapping activity in the eyes, brain, trigeminal ganglia, neural crest, adenohypophysis, sclerotome, and dorsal endoderm. Dse is specifically expressed in the epidermis, anterior surface ectoderm, spinal nerves, notochord and dermatome, whereas Dsel mRNA alone is transcribed in the spinal cord, epibranchial ganglia, prechordal mesendoderm and myotome. The expression of the two genes coincides with sites of cell differentiation in the epidermis and neural tissue. Several expression domains can be linked to previously reported phenotypes of knockout mice and clinical manifestations, such as the Musculocontractural Ehlers-Danlos syndrome and psychiatric disorders.

PubMed ID: 29370293
PMC ID: PMC5784981
Article link: PLoS One

Species referenced: Xenopus laevis
Genes referenced: ag1 dse dsel en2 fn1 foxg1 pax3 snai2 tubb2b twist1
GO keywords: neural crest cell migration [+]

Article Images: [+] show captions
References [+] :
Akatsu, Dermatan sulfate epimerase 2 is the predominant isozyme in the formation of the chondroitin sulfate/dermatan sulfate hybrid structure in postnatal developing mouse brain. 2011, Pubmed