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PLoS One 2013 Jan 01;81:e54777. doi: 10.1371/journal.pone.0054777.
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An intact brachyury function is necessary to prevent spurious axial development in Xenopus laevis.

Aguirre CE , Murgan S , Carrasco AE , López SL .

We have previously shown that the member of the HES family hairy2 induces the ectopic expression of dorsal markers when it is overexpressed in the ventral side of Xenopus embryos. Intriguingly, hairy2 represses the mesoderm transcription factor brachyury (bra) throughout its domain in the marginal zone. Here we show that in early gastrula, bra and hairy2 are expressed in complementary domains. Overexpression of bra repressed hairy2. Interference of bra function with a dominant-negative construct expanded the hairy2 domain and, like hairy2 overexpression, promoted ectopic expression of dorsal axial markers in the ventral side and induced secondary axes without head and notochord. Hairy2 depletion rescued the ectopic dorsal development induced by interference of bra function. We concluded that an intact bra function is necessary to exclude hairy2 expression from the non-organiser field, to impede the ectopic specification of dorsal axial fates and the appearance of incomplete secondary axes. This evidence supports a previously unrecognised role for bra in maintaining the dorsal fates inhibited in the ventral marginal zone, preventing the appearance of trunk duplications.

PubMed ID: 23359630
PMC ID: PMC3554630
Article link: PLoS One

Species referenced: Xenopus laevis
Genes referenced: cd3g cd4 cer1 chrd.1 egr2 en2 foxa4 gsc hes4 mos myc myod1 nodal3.1 not nucb1 otx2 shh sox2 tbxt
Morpholinos: hes4 MO1 hes4 MO4

Article Images: [+] show captions
References [+] :
Amacher, The zebrafish T-box genes no tail and spadetail are required for development of trunk and tail mesoderm and medial floor plate. 2002, Pubmed