Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Dev Biol 2011 Oct 01;3581:240-50. doi: 10.1016/j.ydbio.2011.07.034.
Show Gene links Show Anatomy links

EBF proteins participate in transcriptional regulation of Xenopus muscle development.

Green YS , Vetter ML .

EBF proteins have diverse functions in the development of multiple lineages, including neurons, B cells and adipocytes. During Drosophila muscle development EBF proteins are expressed in muscle progenitors and are required for muscle cell differentiation, but there is no known function of EBF proteins in vertebrate muscle development. In this study, we examine the expression of ebf genes in Xenopus muscle tissue and show that EBF activity is necessary for aspects of Xenopus skeletal muscle development, including somite organization, migration of hypaxial muscle anlagen toward the ventral abdomen, and development of jaw muscle. From a microarray screen, we have identified multiple candidate targets of EBF activity with known roles in muscle development. The candidate targets we have verified are MYOD, MYF5, M-Cadherin and SEB-4. In vivo overexpression of the ebf2 and ebf3 genes leads to ectopic expression of these candidate targets, and knockdown of EBF activity causes downregulation of the endogenous expression of the candidate targets. Furthermore, we found that MYOD and MYF5 are likely to be direct targets. Finally we show that MYOD can upregulate the expression of ebf genes, indicating the presence of a positive feedback loop between EBF and MYOD that we find to be important for maintenance of MYOD expression in Xenopus. These results suggest that EBF activity is important for both stabilizing commitment and driving aspects of differentiation in Xenopus muscle cells.

PubMed ID: 21839736
PMC ID: PMC3352962
Article link: Dev Biol
Grant support: [+]

Species referenced: Xenopus
Genes referenced: acta1 actl6a cdh15 ebf2 ebf3 gal.2 myf5 myod1 nog rbm24 tbxt tnnc1
Antibodies: Somite Ab1
Morpholinos: ebf2 MO1 ebf3 MO1

Article Images: [+] show captions
References [+] :
Anyanful, The RNA-binding protein SUP-12 controls muscle-specific splicing of the ADF/cofilin pre-mRNA in C. elegans. 2004, Pubmed