Cuykendall TN , Houston DW .

5R01 GM083999-02 NIGMS NIH HHS , R01 GM083999-04 NIGMS NIH HHS , R01 GM083999-03 NIGMS NIH HHS , R01 GM083999 NIGMS NIH HHS

	Figure 2. Expression of non-germ plasm localized RNAs. In situ hybridizations for each mRNA, each set of four columns shows stage VI oocytes (left panel), stage 11 embryos (second panel), stage 18 embryos (third panel), and stage 30 embryos (right hand panel). Oocytes and stage 11 embryos are vegetal views; stage 18 embryos are dorsal views, anterior to the left; and stage 30 embryos are lateral views, anterior to the left.
	Figure 3. Expression and germ plasm localization of rtn3.1. In situ hybridization against rtn3.1. A: Stage VI oocyte, vegetal view. B: Two-cell embryo, vegetal view. C: Stage 10, vegetal view. The arrows in A-C indicate rtn3.1 expression in germ plasm. D: Stage 18, anterior view, showing ubiquitous expression and enrichment in the neural plate. E: Stage 24, lateral view, showing enrichment of rtn3.1 in the eye (ey) and branchial arches (ba).
	Figure 4. Expression and germ plasm localization of gptl1. In situ hybridization against gptl1. A: Early stage oocytes (stage I-II, left panel; stage III-IV, right panel). Roman numerals indicate oocyte stages; mc, mitochondrial cloud. B: Stage VI oocyte, vegetal view. C: Four-cell embryo, vegetal view. D: Stage 10.5, vegetal view. E: Stage 30 embryo, cleared in Murray's clear, showing expression in branchial arches and intersomitic boundaries and the absence of staining in primordial germ cells (PGCs). Inset: in situ for xpat showing positive PGC staining in a cleared embryo.
	Figure 6. Expression and germ plasm localization of exd2. In situ hybridization against exd2. A: High magnification view of stage I oocytes; mc, mitochondrial cloud. B: Stage IV (left) and stage VI (right) oocytes, vegetal views. C: Four-cell embryos, vegetal view. D: Stage 18, showing lack of expression. E: Stage 7 sagittal section. F: Stage 11 sagittal section. Insets show low power views in E, F. G: Section of adult testis, exd2 antisense probe. H: Sense control on testis.
	unnamed (hypothetical protein MGC81075) gene expression in Xenopus laevis oocyte, assayed via in situ hybridization, oocyte stage VI, vegetal view.
	pes1 (pescadillo ribosomal biogenesis factor 1) gene expression in Xenopus laevis oocyte, assayed via in situ hybridization, oocyte stages III and IV, vegetal view.
	rtn3 (reticulon 3 ) gene expression in Xenopus laevis oocyte, assayed via in situ hybridization, oocyte stages VI, vegetal view.
	rtn3 (reticulon 3 ) gene expression in Xenopus laevis embryo, assayed via in situ hybridization,NF stage 24, lateral view, dorsal up, anterior left.
	arl8b (ADP-ribosylation factor-like 8B) gene expression in Xenopus laevis embryo, assayed via in situ hybridization,NF stage 30, lateral view, dorsal up, anterior left.
	cab39 (calcium binding protein 39) gene expression in Xenopus laevis embryo, assayed via in situ hybridization,NF stage 30, lateral view, dorsal up, anterior left.
	zfand3 (zinc finger, AN1-type domain 3) gene expression in Xenopus laevis embryo, assayed via in situ hybridization,NF stage 30, lateral view, dorsal up, anterior left.
	midn (midnolin) gene expression in Xenopus laevis embryo, assayed via in situ hybridization,NF stage 30, lateral view, dorsal up, anterior left.
	pced1a (PC-esterase domain containing 1A) gene expression in Xenopus laevis embryo, assayed via in situ hybridization,NF stage 30, lateral view, dorsal up, anterior left.
	triobp (TRIO and F-actin binding protein) gene expression in Xenopus laevis embryo, assayed via in situ hybridization,NF stage 30, lateral view, dorsal up, anterior left.
	exd2 (exonuclease 3'-5' domain containing 2) gene expression in Xenopus laevis embryo, assayed via in situ hybridization, NF stage 3, vegetal view.

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