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Impact of mechanical stress on ion transport in native lung epithelium (Xenopus laevis): short-term activation of Na+, Cl (-) and K+ channels.
Bogdan R
,
Veith C
,
Clauss W
,
Fronius M
.
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Epithelia, in general, and the lungepithelium, in particular, are exposed to mechanical forces, but little is known about their impact on pulmonary ion transport. In our present study, we employed transepithelial ion transport measurements on Xenopus lung preparations using custom-built Ussing chambers. Tissues were exposed to mechanical stress by increasing the water column (5 cm) at one side of the tissues. Apical exposure to hydrostatic pressure significantly decreased the short circuit current (I (SC): 24 +/- 1%, n = 152), slightly decreased the transepithelial resistance (R (T): 7 +/- 2%, n = 152), but increased the apical membrane capacitance (C (M): 16 +/- 6%, n = 9). The pressure-induced effect was sensitive to Na+ (amiloride), Cl(-) (DIDS, NFA, NPPB) and K+ channel blockers (Ba2+), glibenclamide). Further on, it was accompanied by increased extracellular ATP levels. The results show that mechanical stress leads to an activation of Na+, Cl(-), and K+ conductances in a native pulmonary epithelium resulting in a net decrease of ion absorption. This could be of considerable interest, since an altered ion transport may contribute to pathophysiological conditions, e.g., the formation of pulmonary edema during artificial ventilation.
Althaus,
Mechano-sensitivity of epithelial sodium channels (ENaCs): laminar shear stress increases ion channel open probability.
2007, Pubmed,
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Althaus,
Mechano-sensitivity of epithelial sodium channels (ENaCs): laminar shear stress increases ion channel open probability.
2007,
Pubmed
,
Xenbase
Barakat,
A flow-activated chloride-selective membrane current in vascular endothelial cells.
1999,
Pubmed
Brower,
Ventilation with lower tidal volumes as compared with traditional tidal volumes for acute lung injury and the acute respiratory distress syndrome.
2000,
Pubmed
Cavanaugh,
Role of stretch on tight junction structure in alveolar epithelial cells.
2001,
Pubmed
Chatterjee,
KATP channels are an important component of the shear-sensing mechanism in the pulmonary microvasculature.
2006,
Pubmed
Cressman,
Effect of loss of P2Y(2) receptor gene expression on nucleotide regulation of murine epithelial Cl(-) transport.
1999,
Pubmed
Davis,
Two-pore-domain potassium channels support anion secretion from human airway Calu-3 epithelial cells.
2006,
Pubmed
Dawson,
Basolateral K conductance: role in regulation of NaCl absorption and secretion.
1990,
Pubmed
Derdak,
High-frequency oscillatory ventilation for acute respiratory distress syndrome in adult patients.
2003,
Pubmed
Dreyfuss,
Ventilator-induced lung injury: lessons from experimental studies.
1998,
Pubmed
Edwards,
Stretch stimulation: its effects on alveolar type II cell function in the lung.
2001,
Pubmed
Ferguson,
ATP is released from rabbit urinary bladder epithelial cells by hydrostatic pressure changes--a possible sensory mechanism?
1997,
Pubmed
Fischer,
Evidence for apical sodium channels in frog lung epithelial cells.
1989,
Pubmed
,
Xenbase
Fischer,
Regulation of Na+ channels in frog lung epithelium: a target tissue for aldosterone action.
1990,
Pubmed
,
Xenbase
Fitz,
Regulation of cellular ATP release.
2007,
Pubmed
Frank,
Science review: mechanisms of ventilator-induced injury.
2003,
Pubmed
Fronius,
Stimulation of transepithelial Na(+) current by extracellular Gd(3+) in Xenopus laevis alveolar epithelium.
2003,
Pubmed
,
Xenbase
Fronius,
Ion transport across Xenopus alveolar epithelium is regulated by extracellular ATP, UTP and adenosine.
2004,
Pubmed
,
Xenbase
Gao,
Chlorzoxazone or 1-EBIO increases Na(+) absorption across cystic fibrosis airway epithelial cells.
2001,
Pubmed
,
Xenbase
Hamill,
Molecular basis of mechanotransduction in living cells.
2001,
Pubmed
,
Xenbase
Han,
Ventilator-induced lung injury: role of protein-protein interaction in mechanosensation.
2005,
Pubmed
Homolya,
Cell to cell communication in response to mechanical stress via bilateral release of ATP and UTP in polarized epithelia.
2000,
Pubmed
Illek,
Aldosterone regulation of basolateral potassium channels in alveolar epithelium.
1990,
Pubmed
,
Xenbase
Inglis,
A Ba2+-resistant, acid-sensitive K+ conductance in Na+-absorbing H441 human airway epithelial cells.
2007,
Pubmed
Jentsch,
Molecular structure and physiological function of chloride channels.
2002,
Pubmed
Kim,
Active Na+ transport across Xenopus lung alveolar epithelium.
1990,
Pubmed
,
Xenbase
Kitterman,
The effects of mechanical forces on fetal lung growth.
1996,
Pubmed
Krattenmacher,
Electrophysiological analysis of sodium-transport in the colon of the frog (Rana esculenta). Modulation of apical membrane properties by antidiuretic hormone.
1988,
Pubmed
Leipziger,
Control of epithelial transport via luminal P2 receptors.
2003,
Pubmed
Leroy,
Molecular identity and function in transepithelial transport of K(ATP) channels in alveolar epithelial cells.
2004,
Pubmed
Leroy,
Regulation of ENaC and CFTR expression with K+ channel modulators and effect on fluid absorption across alveolar epithelial cells.
2006,
Pubmed
Lewis,
Incorporation of cytoplasmic vesicles into apical membrane of mammalian urinary bladder epithelium.
1982,
Pubmed
Lewis,
Apical membrane area of rabbit urinary bladder increases by fusion of intracellular vesicles: an electrophysiological study.
1984,
Pubmed
Ma,
ATP masks stretch activation of epithelial sodium channels in A6 distal nephron cells.
2002,
Pubmed
Matthay,
Alveolar fluid clearance in patients with ARDS: does it make a difference?
2002,
Pubmed
Meban,
The pneumonocytes in the lung of Xenopus laevis.
1973,
Pubmed
,
Xenbase
Mironov,
Intracellular signalling pathways modulate K(ATP) channels in inspiratory brainstem neurones and their hypoxic activation: involvement of metabotropic receptors, G-proteins and cytoskeleton.
2000,
Pubmed
Nichols,
KATP channels as molecular sensors of cellular metabolism.
2006,
Pubmed
Niemeyer,
Modulation of the two-pore domain acid-sensitive K+ channel TASK-2 (KCNK5) by changes in cell volume.
2001,
Pubmed
Nilius,
Amazing chloride channels: an overview.
2003,
Pubmed
O'Grady,
Chloride and potassium channel function in alveolar epithelial cells.
2003,
Pubmed
Puoti,
The highly selective low-conductance epithelial Na channel of Xenopus laevis A6 kidney cells.
1995,
Pubmed
,
Xenbase
Ricard,
Barotrauma during mechanical ventilation: why aren't we seeing any more?
2004,
Pubmed
Rossier,
Mechanosensitivity of the epithelial sodium channel (ENaC): controversy or pseudocontroversy?
1998,
Pubmed
Sakuma,
Effects of ATP-sensitive potassium channel opener on potassium transport and alveolar fluid clearance in the resected human lung.
1998,
Pubmed
Saumon,
Cellular effects of beta-adrenergic and of cAMP stimulation on potassium transport in rat alveolar epithelium.
1989,
Pubmed
Sheppard,
Mechanism of glibenclamide inhibition of cystic fibrosis transmembrane conductance regulator Cl- channels expressed in a murine cell line.
1997,
Pubmed
Singh,
Stimulation of Cl(-) secretion by chlorzoxazone.
2000,
Pubmed
Sommer,
CFTR-dependent Cl- secretion in Xenopus laevis lung epithelium.
2007,
Pubmed
,
Xenbase
Tarran,
Normal and cystic fibrosis airway surface liquid homeostasis. The effects of phasic shear stress and viral infections.
2005,
Pubmed
Tschumperlin,
Chronic effects of mechanical force on airways.
2006,
Pubmed
Urbach,
Cross-talk between ATP-regulated K+ channels and Na+ transport via cellular metabolism in frog skin principal cells.
1996,
Pubmed
Van Wagoner,
Mechanosensitive gating of atrial ATP-sensitive potassium channels.
1993,
Pubmed
Wang,
IK channels are involved in the regulatory volume decrease in human epithelial cells.
2003,
Pubmed
Ware,
Alveolar fluid clearance is impaired in the majority of patients with acute lung injury and the acute respiratory distress syndrome.
2001,
Pubmed
Welling,
Cross-talk and the role of KATP channels in the proximal tubule.
1995,
Pubmed
Wilson,
Expression of intermediate-conductance, Ca2+-activated K+ channel (KCNN4) in H441 human distal airway epithelial cells.
2006,
Pubmed
Wirtz,
The effects of mechanical forces on lung functions.
2000,
Pubmed
Zünkler,
Concentration-dependent effects of tolbutamide, meglitinide, glipizide, glibenclamide and diazoxide on ATP-regulated K+ currents in pancreatic B-cells.
1988,
Pubmed