Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Methods Enzymol 2014 Jan 01;546:355-75. doi: 10.1016/B978-0-12-801185-0.00017-9.
Show Gene links Show Anatomy links

Cas9-based genome editing in Xenopus tropicalis.

Nakayama T , Blitz IL , Fish MB , Odeleye AO , Manohar S , Cho KW , Grainger RM .

Xenopus tropicalis has been developed as a model organism for developmental biology, providing a system offering both modern genetics and classical embryology. Recently, the Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-associated (CRISPR/Cas) system for genome modification has provided an additional tool for Xenopus researchers to achieve simple and efficient targeted mutagenesis. Here, we provide insights into experimental design and procedures permitting successful application of this technique to Xenopus researchers, and offer a general strategy for performing loss-of-function assays in F0 and subsequently F1 embryos.

PubMed ID: 25398349
PMC ID: PMC4284096
Article link: Methods Enzymol
Grant support: [+]

References [+] :
Abu-Daya, The hitchhiker's guide to Xenopus genetics. 2012, Pubmed, Xenbase