Click here to close Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly. We suggest using a current version of Chrome, FireFox, or Safari.
Biol Open 2012 Dec 15;112:1273-6. doi: 10.1242/bio.20123228.
Show Gene links Show Anatomy links

Highly efficient bi-allelic mutation rates using TALENs in Xenopus tropicalis.

Ishibashi S , Cliffe R , Amaya E .

In the past decade, Xenopus tropicalis has emerged as a powerful new amphibian genetic model system, which offers all of the experimental advantages of its larger cousin, Xenopus laevis. Here we investigated the efficiency of transcription activator-like effector nucleases (TALENs) for generating targeted mutations in endogenous genes in X. tropicalis. For our analysis we targeted the tyrosinase (oculocutaneous albinism IA) (tyr) gene, which is required for the production of skin pigments, such as melanin. We injected mRNA encoding TALENs targeting the first exon of the tyr gene into two-cell-stage embryos. Surprisingly, we found that over 90% of the founder animals developed either partial or full albinism, suggesting that the TALENs induced bi-allelic mutations in the tyr gene at very high frequency in the F0 animals. Furthermore, mutations tyr gene were efficiently transmitted into the F1 progeny, as evidenced by the generation of albino offspring. These findings have far reaching implications in our quest to develop efficient reverse genetic approaches in this emerging amphibian model.

PubMed ID: 23408158
PMC ID: PMC3558749
Article link: Biol Open

Article Images: [+] show captions
References [+] :
Amaya, Frog genetics: Xenopus tropicalis jumps into the future. 1998, Pubmed, Xenbase