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Dev Cell 2015 Nov 09;353:383-94. doi: 10.1016/j.devcel.2015.10.010.
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On the Relationship of Protein and mRNA Dynamics in Vertebrate Embryonic Development.

Peshkin L , Wühr M , Pearl E , Haas W , Freeman RM , Gerhart JC , Klein AM , Horb M , Gygi SP , Kirschner MW .

A biochemical explanation of development from the fertilized egg to the adult requires an understanding of the proteins and RNAs expressed over time during embryogenesis. We present a comprehensive characterization of protein and mRNA dynamics across early development in Xenopus. Surprisingly, we find that most protein levels change little and duplicated genes are expressed similarly. While the correlation between protein and mRNA levels is poor, a mass action kinetics model parameterized using protein synthesis and degradation rates regresses protein dynamics to RNA dynamics, corrected for initial protein concentration. This study provides detailed data for absolute levels of ∼10,000 proteins and ∼28,000 transcripts via a convenient web portal, a rich resource for developmental biologists. It underscores the lasting impact of maternal dowry, finds surprisingly few cases where degradation alone drives a change in protein level, and highlights the importance of transcription in shaping the dynamics of the embryonic proteome.

PubMed ID: 26555057
PMC ID: PMC4776761
Article link: Dev Cell
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: dapl1 ocm rpl11 tnc tnn tpi1

GEO Series: GSE73870: Xenbase,  NCBI
GSE73904: Xenbase,  NCBI
GSE73905: NCBI

Article Images: [+] show captions
References [+] :
Ben-Tabou de-Leon, Modeling the dynamics of transcriptional gene regulatory networks for animal development. 2009, Pubmed