Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Xenopus tropicalis oocytes as an advantageous model system for the study of intracellular Ca(2+) signalling.
Marchant JS
,
Parker I
.
???displayArticle.abstract???
1. The purpose of this study was to compare oocytes from the pipid frogs Xenopus tropicalis and Xenopus laevis, with respect to their utility for studying Ca(2+) signalling mechanisms and for expression of heterologous proteins. 2. We show that X. tropicalis oocytes possess an intracellular Ca(2+) store that is mobilized by inositol (1,4,5) trisphosphate (IP(3)). Ca(2+) signalling is activated by endogenous lysophosphatidic acid receptors and cytosolic Ca(2+) activates a plasma membrane chloride conductance. The spatiotemporal organization of cytosolic Ca(2+) signals, from the microscopic architecture of elementary Ca(2+) 'puffs' to the macroscopic patterns of Ca(2+) spiking are closely similar to the local and global patterns of Ca(2+) release previously characterized in oocytes from X. laevis. 3. By injecting X. tropicalis oocytes with cDNA encoding an ER-targeted fluorescent protein construct, we demonstrate the capacity of the X. tropicalis oocyte to readily express heterologous proteins. The organization of ER is polarized across the oocyte, with the IP(3)-releaseable store targeted within an approximately 8 microm wide band that circumscribes the cell. 4. We conclude that the X. tropicalis oocyte shares many of the characteristics that have made oocytes of X. laevis a favoured system for studying Ca(2+) signalling mechanisms. Moreover, X. tropicalis oocytes display further practical advantages in terms of imaging depth, Ca(2+) signal magnitude and electrical properties. These further enhance the appeal of X. tropicalis as an experimental system, in addition to its greater amenability to transgenic approaches.
Aarhus,
Caged cyclic ADP-ribose. Synthesis and use.
1995, Pubmed
Aarhus,
Caged cyclic ADP-ribose. Synthesis and use.
1995,
Pubmed
Amaya,
Frog genetics: Xenopus tropicalis jumps into the future.
1998,
Pubmed
,
Xenbase
Bereiter-Hahn,
Dynamics of mitochondria in living cells: shape changes, dislocations, fusion, and fission of mitochondria.
1994,
Pubmed
,
Xenbase
Berridge,
Elementary and global aspects of calcium signalling.
1997,
Pubmed
Bezprozvanny,
Inositol (1,4,5)-trisphosphate (InsP3)-gated Ca channels from cerebellum: conduction properties for divalent cations and regulation by intraluminal calcium.
1994,
Pubmed
Bootman,
The elemental principles of calcium signaling.
1995,
Pubmed
Callamaras,
Inositol 1,4,5-trisphosphate receptors in Xenopus laevis oocytes: localization and modulation by Ca2+.
1994,
Pubmed
,
Xenbase
Callamaras,
Hemispheric asymmetry of macroscopic and elementary calcium signals mediated by InsP3 in Xenopus oocytes.
1998,
Pubmed
,
Xenbase
Callamaras,
Radial localization of inositol 1,4,5-trisphosphate-sensitive Ca2+ release sites in Xenopus oocytes resolved by axial confocal linescan imaging.
1999,
Pubmed
,
Xenbase
Callamaras,
Ca(2+)-dependent activation of Cl(-) currents in Xenopus oocytes is modulated by voltage.
2000,
Pubmed
,
Xenbase
Camacho,
Increased frequency of calcium waves in Xenopus laevis oocytes that express a calcium-ATPase.
1993,
Pubmed
,
Xenbase
Campanella,
The modifications of cortical endoplasmic reticulum during in vitro maturation of Xenopus laevis oocytes and its involvement in cortical granule exocytosis.
1984,
Pubmed
,
Xenbase
Cardy,
Differential regulation of types-1 and -3 inositol trisphosphate receptors by cytosolic Ca2+.
1997,
Pubmed
Charbonneau,
The onset of activation responsiveness during maturation coincides with the formation of the cortical endoplasmic reticulum in oocytes of Xenopus laevis.
1984,
Pubmed
,
Xenbase
Colman,
Fate of secretory proteins trapped in oocytes of Xenopus laevis by disruption of the cytoskeleton or by imbalanced subunit synthesis.
1981,
Pubmed
,
Xenbase
Dascal,
The use of Xenopus oocytes for the study of ion channels.
1987,
Pubmed
,
Xenbase
Dawson,
Fire-diffuse-fire model of dynamics of intracellular calcium waves.
1999,
Pubmed
,
Xenbase
DeLisle,
Expression of inositol 1,4,5-trisphosphate receptors changes the Ca2+ signal of Xenopus oocytes.
1996,
Pubmed
,
Xenbase
Dumont,
Oogenesis in Xenopus laevis (Daudin). I. Stages of oocyte development in laboratory maintained animals.
1972,
Pubmed
,
Xenbase
Galione,
Cyclic ADP-ribose: a new way to control calcium.
1993,
Pubmed
Gard,
Ectopic spindle assembly during maturation of Xenopus oocytes: evidence for functional polarization of the oocyte cortex.
1993,
Pubmed
,
Xenbase
Genazzani,
A Ca2+ release mechanism gated by the novel pyridine nucleotide, NAADP.
1997,
Pubmed
,
Xenbase
Gundersen,
Messenger RNA from human brain induces drug- and voltage-operated channels in Xenopus oocytes.
,
Pubmed
,
Xenbase
Gurdon,
Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells.
1971,
Pubmed
,
Xenbase
Kakizawa,
Signaling of lysophosphatidic acid-evoked chloride current: calcium release from inositol trisphosphate-sensitive store.
1998,
Pubmed
,
Xenbase
Kobrinsky,
Expressed ryanodine receptor can substitute for the inositol 1,4,5-trisphosphate receptor in Xenopus laevis oocytes during progesterone-induced maturation.
1995,
Pubmed
,
Xenbase
Kroll,
Transgenic Xenopus embryos from sperm nuclear transplantations reveal FGF signaling requirements during gastrulation.
1996,
Pubmed
,
Xenbase
Kume,
The Xenopus IP3 receptor: structure, function, and localization in oocytes and eggs.
1993,
Pubmed
,
Xenbase
Kusano,
Cholinergic and catecholaminergic receptors in the Xenopus oocyte membrane.
1982,
Pubmed
,
Xenbase
Lechleiter,
Subcellular patterns of calcium release determined by G protein-specific residues of muscarinic receptors.
1991,
Pubmed
,
Xenbase
Lechleiter,
Molecular mechanisms of intracellular calcium excitability in X. laevis oocytes.
1992,
Pubmed
,
Xenbase
Lee,
Caged nicotinic acid adenine dinucleotide phosphate. Synthesis and use.
1997,
Pubmed
Marchant,
Disaccharide polyphosphates based upon adenophostin A activate hepatic D-myo-inositol 1,4,5-trisphosphate receptors.
1997,
Pubmed
Miledi,
Chloride current induced by injection of calcium into Xenopus oocytes.
1984,
Pubmed
,
Xenbase
Nakamura,
Isolation of receptor clones by expression screening in Xenopus oocytes.
1999,
Pubmed
,
Xenbase
Parker,
Nonlinearity and facilitation in phosphoinositide signaling studied by the use of caged inositol trisphosphate in Xenopus oocytes.
1989,
Pubmed
,
Xenbase
Parker,
Regenerative release of calcium from functionally discrete subcellular stores by inositol trisphosphate.
1991,
Pubmed
,
Xenbase
Parker,
Caffeine inhibits inositol trisphosphate-mediated liberation of intracellular calcium in Xenopus oocytes.
1991,
Pubmed
,
Xenbase
Parker,
Elementary events of InsP3-induced Ca2+ liberation in Xenopus oocytes: hot spots, puffs and blips.
1996,
Pubmed
,
Xenbase
Parker,
A high-resolution, confocal laser-scanning microscope and flash photolysis system for physiological studies.
1997,
Pubmed
,
Xenbase
Parys,
Isolation, characterization, and localization of the inositol 1,4,5-trisphosphate receptor protein in Xenopus laevis oocytes.
1992,
Pubmed
,
Xenbase
Parys,
The inositol trisphosphate receptor of Xenopus oocytes.
1995,
Pubmed
,
Xenbase
Streb,
Release of Ca2+ from a nonmitochondrial intracellular store in pancreatic acinar cells by inositol-1,4,5-trisphosphate.
,
Pubmed
Sun,
A continuum of InsP3-mediated elementary Ca2+ signalling events in Xenopus oocytes.
1998,
Pubmed
,
Xenbase
Taylor,
Pharmacological analysis of intracellular Ca2+ signalling: problems and pitfalls.
1998,
Pubmed
Theodoulou,
Xenopus oocytes as a heterologous expression system.
1995,
Pubmed
,
Xenbase
Walker,
Kinetics of smooth and skeletal muscle activation by laser pulse photolysis of caged inositol 1,4,5-trisphosphate.
,
Pubmed
Yao,
Quantal puffs of intracellular Ca2+ evoked by inositol trisphosphate in Xenopus oocytes.
1995,
Pubmed
,
Xenbase