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XB-ART-44652
Methods Mol Biol 2012 Jan 01;839:119-31. doi: 10.1007/978-1-61779-510-7_10.
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Activation and function of small GTPases Rho, Rac, and Cdc42 during gastrulation.

Mezzacappa C , Komiya Y , Habas R .


Abstract
Gastrulation is comprised of a series of cell polarization and directional cell migration events that establish the physical body plan of the embryo. One of the major ligand-based pathways that has emerged to play crucial roles in the regulation of gastrulation is the non-canonical Wnt signaling pathway. This aspect of Wnt signaling is comprised of a number of signaling branches that are subsequently integrated for the regulation of changes to the actin cytoskeleton during cell polarization and cell migration during vertebrate gastrulation. The Rho family of small GTPases are activated and required for non-canonical Wnt signaling during gastrulation, and in this chapter, we describe biochemical assays for the detection of Wnt-mediated activation of Rho, Rac, and Cdc42 in both mammalian cells and Xenopus embryo explants.

PubMed ID: 22218897
PMC ID: PMC4414490
Article link: Methods Mol Biol
Grant support: [+]

Species referenced: Xenopus laevis
Genes referenced: actl6a akt1 cdc42 rac1 rho rho.2

References [+] :
Akasaki, Phosphoinositide 3-kinase-dependent and -independent activation of the small GTPase Rac2 in human neutrophils. 1999, Pubmed