Summary |
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Synonym: |
pDPCrtTA-TREG-HS4; HS4-CMV:rtTA/TRE:eGFP/Cry:GFP3-HS4 |
Species: |
Xenopus laevis |
Background Strain: |
Xla.Outbred
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Paternal Line: |
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Maternal Line: |
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Description: |
Transgenesis construct has the Dox-activated transcription factor rtTA under the control of the ubiquitous promoter CMV and green fluorescent protein GFP is regulated by the modified tetracycline response element TREtight. Construct also has a crystallin:GFP expression cassette for constitutive expression of GFP in the lens as a marker of transgenesis and two directly repeated HS4 insulator elements and I Sce-I sides.
Both CMV:rtTA and TRE:GFP cassettes were included on the same transgenesis construct so expression levels and uniformity of Dox-induced GFP in the F0 tadpoles could be evaluated
Treatment with 50ug/mL doxycycline for 1 day at NF45 will induce ubiquitous GFP expression. Constitutive GFP expression in eye from crystallin:GFP cassette.
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Phenotype Description: |
DOX inducible ubiquitous GFP expression, and GFP expression in lens. |
Color Morph: |
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Breeding Type: |
UNKNOWN
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Lab of Origin: |
Buchholz Lab |
Line Type: |
Transgenic |
Mutated Gene(s): |
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MTA Required: |
No
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Public: |
Yes
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Catalogue Number: |
EXRC Line #50 |