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XB-LINE-794
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Line Name: Xla.Tg(hsp70:nog,cryga:GFP)Jmws
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| Summary | |
|---|---|
| Synonym: | HGEM Noggin; HSP70-Noggin-gCrys-GFP; heat shock green-eyed monster |
| Species: | Xenopus laevis |
| Background Strain: | Xla.Outbred |
| Paternal Line: | |
| Maternal Line: | |
| Description: | Heat shock inducible Noggin line. Noggin (nog) under control of HSP70, and gamma Crystalllin-GFP double transgenic. HGEM vector (heat shock green-eyed monster) contains a Xenopus HSP70 promoter (Marsh-Armstrong et al., 1999) upstream of a multicloning site (MCS), followed by a 6x myc tag and SV40 poly A, and the Xenopus gamma-crystallin promoter driving expression of GFP (from Rob Grainger's lab) as a visible marker for transgene insertion. |
| Phenotype Description: | If transgenic embryos are heat shocked at stage 14 they develop multiple defects consistent with BMP inhibition, and expression of GFP in lens. |
| Color Morph: | |
| Breeding Type: | OUTBRED |
| Lab of Origin: | Slack Lab |
| Line Type: | Transgenic |
| Mutated Gene(s): | |
| MTA Required: | Yes |
| Public: | Yes |
| Catalogue Number: | EXRC line #18 |
| Stock Center | RRID | Generation | Availability | Mutant Details | |
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| NXR (US) | NXR_0020 | F2 | Order | ||
| EXRC (Europe) | EXRC_0018 | ||||
| NBRP (Japan) | |||||
| XLRRI (US) | |||||
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