XB-LINE-2394
Line Name: Xla.outbredXen1
| Summary |
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| Species: |
Xenopus laevis |
| Background Strain: |
Unknown
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| Description: |
X. laevis outbred from Xenopus One, a commercial supplier of Xenopus frogs. |
| Phenotype Description: |
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| Color Morph: |
pigmented |
| Breeding Type: |
OUTBRED
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| Line Type: |
Wild Type |
| Mutated Gene(s): |
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| MTA Required: |
No
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| Public: |
Yes
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Fig. 5. Depletion of Dg affects laminin-1 assembly and tubule formation in vitro. Two-cell stage embryos were injected with Dg-Mo1 + 2 (32 ng/embryo). Animal caps were treated with activin/retinoic acid for 3 h. (A, D) Immunodetection with the 3G8 antibodies and the anti-laminin antibodies. (A, B) Section of a control animal cap. 3G8 (blue arrowhead) and anti-laminin-1 (red arrowhead) antibodies staining are detected. Laminin-1 is present at the basal pole of cells of the tubules. Laminin-1 is also observed elsewhere in the explant where the tubules will be formed (purple arrowhead) (A). (C, D) Section of a Mo1 + 2 injected explant (C). Large cavities and mesenchyme like tissues are observed. (D) A diffuse fluorescence is observed (white arrowhead) suggesting that laminin-1 is accumulated but does not assemble basal lamina. (E–H) Expression of pronephros marker genes in animal caps. (E, G) The expression of the Pax-8 gene is similar in the control and Mo1 + 2 injected explant. (F, H) Pax-2 gene is detected in control and Mo1 + 2 injected explant.
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| Stock Center |
RRID |
Generation |
DOB |
# Males |
# Females |
Availability |
Mutant Details |
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No availability, contact the Lab of Origin for information about this Xenopus line or strain. Articles using these frogs are listed on the Attribution Tab.
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