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Figure 2. muzak is encoded by myh6 WISH shows myh6 expression in wild type heart (A, black arrow) and jaw muscle (white arrow) is diminished in muz (B). (C, D) myh6.2 is expressed in jaw muscle (white arrow) but not heart (black arrow), and is unaffected by the mutation. (E)
Schematic showing domain structure of wild type X. tropicalis myh6 and the truncated protein lacking the myosin coiled-coil tail encoded by the muz allele. (F) Western blot analysis does not detect sarcomeric MHC protein in extracts of muz heart; silver stained loading control below. (Movie S2 and G) myh6 morphant hearts do not beat and show strong
depletion of sarcomeric MHC protein relative to control morpholino-injected tadpoles; silver stained loading control below.
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hba1 (hemoglobin subunit alpha 1) gene expression in Xtr.myh6 mutant tadpole, ventral view, anterior left. This mutant has impaired in heart function but normal hematopoiesis, leading to ventral pooling and normal levels of blood (white arrows)
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