Summary Attributions Transgene

Line Name: Xla.Tg(hsp70:dkk1-GFP;tdTomato)Tamur

Line Images [+] show captions [-] hide captions Map of the heat-shock-inducible Dkk1GFP transgene with lens labeling. The bright RFP variant, tdTomato under control of the 2.2-kb γ–crystallin promoter, was introduced downstream of hsp70-Dkk1GFP. Map of the heat-shock-inducible Dkk1GFP transgene with lens labeling. The bright RFP variant, tdTomato under control of the 2.2-kb γ–crystallin promoter, was introduced downstream of hsp70-Dkk1GFP. hsDkk1 inhibited the forelimb bud regeneration in tadpoles. (A and B) Dorsal view of the left forelimb bud at stage 54. A paddle-shaped forelimb bud can be seen in a cavity dorsal to the posterior portion of the gill region (A). The hsDkk1GFP expression was induced in the entire tadpole body, including the forelimb bud region, by heat-shock (B).

Summary
Synonym:	hsp70:Dkk1GFP, gCry:tdTomato
Species:	Xenopus laevis
Background Strain:	Xla.Outbred
Paternal Line:
Maternal Line:
Description:	30 bp of promoter of the X. laevis hsp70 gene driving expression Dkk1GFP and 2.2 kb gamma crystallin promoter driving tdTomato. Dkk1 (labeled withGFP) specifically blocks Wnt/beta-catenin signaling and Dkk1GFP expression can be induced at a specific time-point by a heat-shock in this transgenic Xenopus. Thus, you can block Wnt/beta-catenin signaling temporally at desired time-point by a heat-shock. Transgenic X. laevis embryos were generated by a modified REMI technique using oocyte extract instead of egg extract.
Phenotype Description:	dkk1-GFP expression induced temporally via heat shock, and red fluorescence in lens.
Color Morph:	pigmented
Breeding Type:	UNKNOWN
Lab of Origin:	Tamura Lab
Line Type:	Transgenic
Mutated Gene(s):
MTA Required:	No
Public:	Yes
Catalogue Number:	EXRC line 79#, waiting for EXRC RRID

Stock Center	RRID	Generation	Availability	Mutant Details
NXR (US)
EXRC (Europe)
NBRP (Japan)
XLRRI (US)

Back to Search Lines