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XB-LINE-984
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Line Name: Xla.Tg(Xtr.col2a1:RTTA;TRE:Cre;cryga:GFP3)Buchz
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| Summary | |
|---|---|
| Synonym: | pDPcol2.3rtTA-TRECre-HS4 |
| Species: | Xenopus laevis |
| Background Strain: | Xla.Outbred |
| Paternal Line: | |
| Maternal Line: | |
| Description: | This inducer line contains the chondrocyte-specific regulatory elements from the 5' region and first intron of the Xenopus tropicalis col2a1 gene (Kerney et al. 2010). These activate the tetracycline/doxycycline conditional transcription factor rtTA, which in the presence of doxycycline (Dox), induces expression of Cre-recombinase (Cre) via the tetracycline/ doxycycline response element (TRE) promoter in our transgenic line. The spatial expression of rtTA is restricted to cartilage by col2a1 regulatory elements. Temporal control of TRE activation by rtTA depends on the addition of Dox to the rearing medium. |
| Phenotype Description: | Used with transgenic reporter frogs to inducibly label cartilage. Constitutive GFP expression in eye from crystallin:GFP cassette. No other visible phenotype in the absence of a cross with a transgenic frog harboring a reporter transgene |
| Color Morph: | |
| Breeding Type: | UNKNOWN |
| Lab of Origin: | Buchholz Lab |
| Line Type: | Transgenic |
| Mutated Gene(s): | |
| MTA Required: | No |
| Public: | Yes |
| Catalogue Number: | EXRC line #51 |
| Stock Center | RRID | Generation | Availability | Mutant Details |
|---|---|---|---|---|
| NXR (US) | ||||
| EXRC (Europe) | EXRC_0051 | |||
| NBRP (Japan) | ||||
| XLRRI (US) | ||||
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