| Summary |
|
| Synonym: |
pDPCrtTA-TREG-HS4; HS4-CMV:rtTA/TRE:eGFP/Cry:GFP3-HS4 |
| Species: |
Xenopus laevis |
| Background Strain: |
Xla.Outbred
|
| Paternal Line: |
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| Maternal Line: |
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| Description: |
Transgenesis construct has the Dox-activated transcription factor rtTA under the control of the ubiquitous promoter CMV and green fluorescent protein GFP is regulated by the modified tetracycline response element TREtight. Construct also has a crystallin:GFP expression cassette for constitutive expression of GFP in the lens as a marker of transgenesis and two directly repeated HS4 insulator elements and I Sce-I sides.
Both CMV:rtTA and TRE:GFP cassettes were included on the same transgenesis construct so expression levels and uniformity of Dox-induced GFP in the F0 tadpoles could be evaluated
Treatment with 50ug/mL doxycycline for 1 day at NF45 will induce ubiquitous GFP expression. Constitutive GFP expression in eye from crystallin:GFP cassette.
|
| Phenotype Description: |
DOX inducible ubiquitous GFP expression, and GFP expression in lens. |
| Color Morph: |
|
| Breeding Type: |
UNKNOWN
|
| Lab of Origin: |
Buchholz Lab |
| Line Type: |
Transgenic |
| Mutated Gene(s): |
|
| MTA Required: |
No
|
| Public: |
Yes
|
| Catalogue Number: |
EXRC Line #50 |
