Figure 4. Characterization of the Foxn1-knockout tadpoles. (A) The target sites of anti-Foxn1 TALEN in Foxn1 genomic gene and Foxn1 protein. The alignment is labeled as described in the legend to Fig. 2. (B) Photographs of heads of stage 56 wild-type and Foxn1-knockout tadpoles. Arrowheads indicate the thymus in the wild-type tadpole. There is no thymus in the Foxn1-knockout tadpole. Scale bars = 3 mm. (C) Flow cytofluorometric analysis of splenocytes from wild-type and Foxn1-knockout frogs after staining with mouse anti-CD8 monoclonal antibody (AM22 or F17) and Alexa Fluor 488 goat anti-mouse IgM antibody. (D) Transplantation of ventral skin grafts from wild-type Nigerian H (NH) and Ivory Coast (IC) strains to the backs of IC wild-type and Foxn1-knockout frogs. Note that both grafts survived on the back of Foxn1-knockout frog for more than 100 days. Scale bars = 1 mm.