| Description: |
One-cell stage Xenopus Xla.Tg(Cardiac-actin:GFP)Mohun embryos were microinjected with 1 ng TALEN mRNA to induce mutations in kcp, Mutant tadpoles were grown to adulthood and crossed with wild-type or Xla.Tg(Cardiac-actin:GFP)Mohun frogs to generate F1 embryos and assess germline transmission of kcp locus mutations. Kcp mutant founders were subsequently confirmed and mated to generate F1 compound heterozygous Kcp mutant embryos.All mutant animals ... were PCR-genotyped and subsequently sequenced to verify the incorporation of mutations into both kcp alleles resulting in a frame-shift and premature stop codon in the LaminG protein domain, prior to the essential cysteine-rich elements. |
