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Xine Volume 11 - number 2, February 2011

Welcome to Xine, the source for Xenopus news and information. Here's
what's happening...

(from Gerry Thomsen and Amy Sater)

We still have a few openings for the Xenopus Course at CSHL this April, so we encourage you to bring this opportunity to the attention of anyone in your lab who would benefit from this experience.


APRIL 8 - 19, 2011

Application deadline: February 25

2011 Instructors:
Amy Sater, University of Houston
Gerald Thomsen, Stony Brook University

Thanks to HHMI and federal funds, substantial scholarships towards tuition, room and board are available based on stated need (include stipend request with application). Individuals from US labs/US citizens are particularly encouraged to apply.

Please pass this along to colleagues or members of your laboratory or group who may benefit from this training. A diverse faculty will bring the most up-to-date results and theories to the students, making this course a valuable resource for young researchers starting out in this fast-moving and expansive field.

Xenopus is the leading vertebrate model for the study of gene function in development. The combination of lineage analysis, gene-knockout strategies, experimental manipulation of the embryo, and genomic/bioinformatic techniques, makes it ideal for studies on the molecular control of embryo patterning, morphogenesis and organogenesis. The course combines intensive laboratory training with daily lectures from recognized experts in the field. Students will learn both emerging technologies and classical techniques to study gene function in Xenopus development. An important element will be the informal interaction between students and course faculty.

Technologies to be covered will include: oocyte and embryo culture, lineage analysis and experimental manipulation of embryos, time lapse imaging of morphogenesis, gain and loss of function analysis using mRNAs and antisense oligos, analysis of regeneration, whole mount in situ hybridization, immunocytochemistry, RT-PCR, and genomic/bioinformatic techniques, preparation of transgenic embryos, and use of Xenopus tropicalis for genetic analyses. The Cell and Developmental Biology of Xenopus course is designed for those new to the Xenopus field, as well as for those wanting a refresher course in the emerging technologies. The course is open to investigators from all countries.

Lecturers for the 2011 course will include:

Rob Grainger

Richard Harland

Doug Houston

Ray Keller

Mustafa Khokha

Kris Kroll

Kevin Lin (Jonathan Slack's lab)

Amy Sater

Jerry Thomsen

John Wallingford

Lyle Zimmerman
Call for content

Xine could be used to disseminate information and
protocols of general utility to the research community. In order for
this to occur, please send any such contributions to the editor who
will include them in a future (or special) issue of Xine.
If you wish to read Xine in html format and/or see back issues,
they are available at the following places
Links to useful sources of information for Xenopus (in no particular order)

general interest and utility
<> Trans NIH Xenopus
<> - Harland lab X. tropicalis site
<> - Grainger lab X. tropicalis site
<> - Amy Sater's X. tropicalis genetic map
<> - Information on the
X. tropicalis listserver
<> - Troplist archives.
Lots of good information here.
<> - Peter Vize's Xenopus Uber database
<> - Zimmerman Lab
X. tropicalis website, database of mutants
<> - Shoko Ishibashi's trop web page

genomic resources
<> - XDB at NIBB - Naoto Ueno's
X. laevis EST database <> - Xenopus gene collection
<> - full length
collection at the Gurdon Institute
<> - JGI X. tropicalis
genome site with browser and other info
Subscription information

I have constructed the Xine mailing list from serveral sources. As
always, if you are not on the list and wish to be, want to update your
e-mail address or would rather not receive it at all, please contact
Bruce Blumberg (

Until next time,