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XB-PHENO-23998

Xla Wt + ctnnb1 MO, Colozza G and De Robertis EM (2020)

Experiment + Assay: Xla Wt + ctnnb1 MO + NF10 (RT-PCR)
Background: Xla.pigmentedNasco
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection ctnnb1 MO1 ctnnb1.L
NF stage 4 (8-cell) vegetal blastomere
Conditions:
Assay Type Assay Stages Antibody
RT-PCR NF stage 10 to NF stage 10  
Expression Phenotypes (2):
absent chd1.L expression in upper blastopore lip
decreased amount dact4.L expression in upper blastopore lip
Source: Fig. 4C
Article: XB-ART-57524, PubMed