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XB-PHENO-23207

Xla Wt + sia1-GR + CHX + DEX + animal cap explant, Rankin SA et al. (2011)

Experiment + Assay: Xla Wt + sia1-GR + CHX + DEX + animal cap explant + NF11 (in situ hybridization)
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
mRNA injection Tg(sia1.L-GR) sia1.S
sia1.L
NF stage 5 (16-cell) ventral vegetal hemisphere blastomere
explant  
blastula stage animal cap
chemical dexamethasone
blastula stage animal cap
chemical cycloheximide
blastula stage animal cap
Conditions:
Assay Type Assay Stages Antibody
in situ hybridization NF stage 11  
Expression Phenotypes (5):
increased amount chrd.1.L expression in animal cap
decreased amount hhex.L expression in animal cap
increased amount lhx1.L expression in animal cap
increased amount otx2.L expression in animal cap
increased amount ventx1.2 expression in animal cap
Source: fig.7.a
Article: XB-ART-42631, PubMed