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XB-PHENO-22923

Xla Wt + sia1-GR + ventx1.1 MO + CHX + DEX + animal cap explant, Rankin SA et al. (2011)

Experiment + Assay: Xla Wt + sia1-GR + ventx1.1 MO + CHX + DEX + animal cap explant + NF11 (in situ hybridization)
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection ventx1.1 MO1 ventx1.L
ventx1.S
 NF stage 5 (16-cell) animal blastomere
mRNA injection Tg(sia1.L-GR) sia1.S
sia1.L
 NF stage 5 (16-cell) animal blastomere
chemical dexamethasone
blastula stage animal cap
explant  
blastula stage animal cap
chemical cycloheximide
blastula stage animal cap
Conditions:
Assay Type Assay Stages Antibody
in situ hybridization NF stage 11  
Expression Phenotypes (2):
increased amount chrd.1.L expression in animal cap
increased amount hhex.L expression in animal cap
Source: fig.9.c
Article: XB-ART-42631, PubMed