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XB-PHENO-22026

Xla Wt + flop2 MO + mgc69520 MO, Miyagi A et al. (2015)

Experiment + Assay: Xla Wt + flop2 MO + mgc69520 MO + NF17-18 (in situ hybridization)
Background: Xla.Outbred
Manipulations:
Manipulation Type Reagent Target Gene Stage Treatment Area
morpholino injection mgc69520 MO2 gpr4l.2.S
gpr4l.2.L
 NF stage 3 (4-cell) blastomere
morpholino injection flop2 MO1 gpr4l.S
gpr4l.L
 NF stage 3 (4-cell) blastomere
Conditions:
Assay Type Assay Stages Antibody
in situ hybridization NF stage 17 to NF stage 18  
Expression Phenotypes (2):
decreased amount en2.L expression in presumptive midbrain-hindbrain boundary
decreased amount foxg1.L expression in anterior neural ridge
Source: fig.2.c
Article: XB-ART-51082, PubMed