Fig. 1 C

Lichtig H et al. (2020)

Xla + asxl3 MO

NF16 (RT-PCR)

Expression Phenotype
decreased amount egr2.L expression in embryo decreased amount tubb2b.L expression in embryo

Fig. 3 B lanes 4,5

Lichtig H et al. (2020)

Xla + asxl3 MO

NF16 (RT-PCR)

Expression Phenotype
decreased amount egr2.L expression in embryo decreased amount hoxb1.L expression in embryo decreased amount hoxb3.L expression in embryo

Fig. 4 C lane 3

Lichtig H et al. (2020)

Xla + asxl3 MO

NF16 (RT-PCR)

Expression Phenotype
absent ag1.L expression in embryo absent egr2.L expression in embryo absent foxd1.L expression in embryo absent foxd3.L expression in embryo absent foxg1.L expression in embryo absent tubb2b.L expression in embryo decreased amount hoxb3.L expression in embryo decreased amount ncam1.L expression in embryo decreased amount snai1.L expression in embryo

Fig. 2 D 2rc2

Lichtig H et al. (2020)

Xla + asxl3 MO

NF17-19 (whole-mount microscopy)

Expression Phenotype
decreased amount twist1.L expression in cranial neural crest decreased amount twist1.L expression in neural tube

Fig. 1 B r1c2

Lichtig H et al. (2020)

Xla + asxl3 MO

NF18 (in situ hybridization)

Anatomical Phenotype
abnormal anterior neural tube morphology abnormal neural tube closure

Expression Phenotype
decreased amount tubb2b.L expression in neural tube decreased amount tubb2b.L expression in Rohon-Beard neuron decreased amount tubb2b.L expression in trigeminal placode

Fig. 1B r2c2

Lichtig H et al. (2020)

Xla + asxl3 MO

NF18 (in situ hybridization)

Expression Phenotype
absent egr2.L expression in hindbrain absent egr2.L expression in rhombomere R3 absent egr2.L expression in rhombomere R5 decreased amount egr2.L expression in cranial neural crest

Fig. 4 A lane 5

Lichtig H et al. (2020)

Xla Wt + asxl3 MO + animal cap amputation

NF16 (RT-PCR)

Expression Phenotype
absent ag1.L expression in animal cap absent foxd1.L expression in animal cap absent foxg1.L expression in animal cap absent hesx1.L expression in animal cap absent ncam1.L expression in animal cap absent otx2.L expression in animal cap

Fig. 4 B lane 6

Lichtig H et al. (2020)

Xla Wt + asxl3 MO + animal cap amputation

NF16 (RT-PCR)

Expression Phenotype
absent ncam1.L expression in animal cap absent otx2.L expression in animal cap absent tubb2b.L expression in animal cap

Fig. 3 A lane 6

Lichtig H et al. (2020)

Xla Wt + meis3 + asxl3 MO

NF16 (RT-PCR)

Expression Phenotype
decreased amount egr2.L expression in animal cap decreased amount hoxd1.L expression in animal cap