Fig. 2. Inhibition of miRNA biogenesis affects patterning of the pronephros. (A-G′) Whole-mount in situ hybridization for markers of terminal pronephros differentiation on uninjected and Dgcr8-MO-injected Xenopus embryos at stage 39. (A,A′) SGLT1-K; (B,B′) NKCC2; (C,C′) NBC1; (D,D′) ClC-K; (E,E′) Cadherin-16; (F,F′) ROMK; (G,G′) NCC. Arrowheads indicate a reduction in the intermediate and distal tubular domain. (H,I) Schematics illustrating the different pronephric regions and their corresponding marker gene expression (H) and the phenotype of Dicer-MO- or Dgcr8-MO-injected embryos showing a reduction in the proximal and intermediate tubules as well as in distal tubule DT1 (I).
Image published in: Agrawal R et al. (2009)
Copyright © 2009. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| slc5a1.2.S | D22S675, sglt-1, sglt-1k, sglt1 | X. laevis | Throughout NF stage 39 | early proximal tubule late proximal tubule |
| slc12a1.L | Na-K-2Cl cotransporter, NKCC-2, NKCC2, XNKCC2 | X. laevis | Throughout NF stage 39 | early distal tubule late distal tubule |
| slc4a4.L | hhNMC, hnbc1, nbc1, nbc2, nbce1, pNBC, slc4a5, XNBC1 | X. laevis | Throughout NF stage 39 | early distal tubule late distal tubule |
| clcnkb.L | ClC-k, clc-kb, clckb, clcnka, clk-k2, xClC-k | X. laevis | Throughout NF stage 39 | early distal tubule late distal tubule pronephric duct |
| cdh16.L | cadherin-16, KSP-cadherin | X. laevis | Throughout NF stage 39 | early distal tubule late distal tubule |
| kcnj1.L | kir1.1, MGC68935, ROMK, romk1 | X. laevis | Throughout NF stage 39 | early distal tubule pronephric duct kidney |
| slc12a3.L | ncc, ncct, tsc | X. laevis | Throughout NF stage 39 | early distal tubule late distal tubule pronephric duct |
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