Fig. 1. Xenopus laevis Endothelin-1 receptor type A features and expression pattern. (A) Molecular phylogenetic analysis of Ednra shows the relationship with Xenopus ETAX, Xenopus Ednrb and with Ednra from different vertebrate species. The protein sequences of different Ednra were analyzed using ClustalW 1.81, and an unrooted tree was constructed by neighbour-joining analysis. Xenopus laevis Ednrb, Danio rerio EdnrA1, and Danio rerio EdnrA2 branches are collapsed 50%. (B) RT-PCR analysis of temporal expression pattern of Ednra, Ppet-1, and ECE-1 during different developmental stages. Total RNA isolated from stages 1, 10.5, 12, 14, 18, and 21 was isolated, retrotranscribed and amplified as described in Materials and methods. Contamination of genomic DNA was examined by experiments without RT reactions (−RT) using total RNA from stage 18 embryos. EF1α expression was used as loading control. (C–L) Expression pattern of Xenopus laevis Ednra. (C) Ednra transcripts are first detected from late gastrula stage (St. 12.5) in the lateral domains of neural plate (arrows). (D) During neurulation Ednra is expressed in the prospective cephalic (asterisk) and trunk neural crest (arrows). (E) In situ hybridization for FoxD3. (F) Double in situ hybridization for Ednra (purple) and Sox2 (turquoise). (G) Transverse section of (F). Asterisk, prospective neural crest. (H) Tailbud-stage (St. 24) embryo showing expression in the migrating cephalic neural crest (arrowheads), trunk region (arrow), crest cells migration into dorsal fin (white arrowheads), and otic vesicle (OtV). (I) Frontal section of (H), Ednra expression in the otic vesicle (OtV) and the neural crest hyoid migratory stream (arrowheads). NT, neural tube; n, notochord. (J) A St. 30-embryo showing Ednra expression in the branchial arches (black arrowheads), cells into the dorsal fin (white arrowheads), and otic vesicle (OtV). OpV, optic vesicle. (K) Section showing Ednra expression in the dorsomedial and ventromedial region of the otic vesicle (arrowheads). (L) Horizontal section showing Ednra expression (arrowheads) in the first and second pharyngeal arches surrounding the central mesodermal core (asterisks).
Image published in: Bonano M et al. (2008)
Copyright © 2008. Image reproduced with permission of the Publisher, Elsevier B. V.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| sox2.L | anop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2 | X. laevis | Throughout NF stage 15 | neural plate neuroectoderm |
| ednra.L | ednra-a, ednra-b, endo-r, Endothelin-1 receptor type A, eta, etar, etax, etra | X. laevis | Throughout NF stage 12.5 | ectoderm neural plate border |
| foxd3.L | fkh6, foxd3-a, foxd3-b, XFD-6, xfoxd3 | X. laevis | Throughout NF stage 15 | neural crest neuroectoderm |
| ednra.L | ednra-a, ednra-b, endo-r, Endothelin-1 receptor type A, eta, etar, etax, etra | X. laevis | Throughout NF stage 15 | trunk neural crest trunk neural crest dorsal neural plate border |
| ednra.L | ednra-a, ednra-b, endo-r, Endothelin-1 receptor type A, eta, etar, etax, etra | X. laevis | Throughout NF stage 24 | neural crest otic vesicle trunk neural crest dorsal tail fin hyoid crest migratory neural crest cell cranial neural crest mandibular crest anterior branchial crest posterior branchial crest periocular region |
| ednra.L | ednra-a, ednra-b, endo-r, Endothelin-1 receptor type A, eta, etar, etax, etra | X. laevis | Throughout NF stage 29 and 30 | otic vesicle dorsal tail fin branchial arch pharyngeal arch mandibular arch neural crest trunk neural crest dorsal fin intersomitic region periocular region cranial neural crest mandibular crest hyoid crest anterior branchial crest posterior branchial crest |
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