Larger Image

Fig. 2. Expression patterns of XPIASy and XSmad2 during Xenopus development. (A) Semi-quantitative RT-PCR analysis of XPIASy and XSmad2 expression was performed using whole embryos at different stages. ODC (Ornithine decarboxylase) was used as a control. (B) XPIASy and XSmad2 show different temporal expression in the marginal zone at the early gastrula stage. Stage 10 embryos were dissected as shown into animal pole (AN), vegetal pole (VG), DMZ (D) or VMZ (V) explants, or whole embryos (WE), and subjected to RT-PCR analysis of XPIASy and XSmad2. XPIASy is expressed strongly in the VMZ compared with the DMZ. (C) In situ hybridization of XPIASy (a-g) and XSmad2 (h-n) during development. (a,h) Stage 6-1/2, lateral view; (b,i) stage 10; (c,j) stage 18, anterior view; (d,k) stage 25; (e,l) stage 27; (f,m) stage 29/30; and (g,n) stage 37/38.

Image published in: Daniels M et al. (2004)

Copyright © 2004. Image reproduced with permission of the publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.

GeneSynonymsSpeciesStage(s)Tissue
pias4.Lpiasg, piasy, XPIASy, zmiz6X. laevisThroughout NF stage 6.5animal hemisphere
pias4.Lpiasg, piasy, XPIASy, zmiz6X. laevisThroughout NF stage 18anterior
eye primordium
cranial neural crest
neural plate border
pre-chordal neural plate
pias4.Lpiasg, piasy, XPIASy, zmiz6X. laevisSometime during NF stage 25 to NF stage 37 and 38eye
neural tube
pharyngeal arch
otic placode
paraxial mesoderm
presomitic mesoderm
smad2.Ssmad-2, XSmad2X. laevisThroughout NF stage 6.5animal hemisphere
smad2.Ssmad-2, XSmad2X. laevisSometime during NF stage 10 to NF stage 18neuroectoderm
anterior
eye primordium
smad2.Ssmad-2, XSmad2X. laevisSometime during NF stage 25 to NF stage 28neural tube
eye
pharyngeal region
otic vesicle
paraxial mesoderm
tail bud
smad2.Ssmad-2, XSmad2X. laevisThroughout NF stage 37 and 38head
brain
eye
pharyngeal arch
spinal cord
tail
somite

Image source: Published

Permanent Image Page
Printer Friendly View

XB-IMG-2343