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Fig. 5. Normal expression of stem cell (rx1, pax6, six3), neural identity (notch1, sox2) and differentiation (ngn1, p27, neuroD, Xath5) markers in control embryos. Embryos injected with β-gal mRNA were processed by in situ hybridization to determine the normal expression pattern of each marker gene. All stages 22–27 embryos are external views of whole-mount preparations [bars = 100 μm]; all stage 38 views are from whole mount preparations that were thickly sectioned to reveal internal expression patterns [bar = 20 μm]. (A) Summary of the expression domains of marker genes at stage 38 in the different layers of the retina, based on data from Perron and Harris (1999), Ohnuma et al. (1999) and our results. All genes except ngn1 are expressed in some part of the ciliary marginal zone (CMZ). Only pax6, rx1, six3 and sox2 are expressed in all zones of the CMZ. Other genes are expressed only in the zones of the CMZ (2, 3) closest to the central retina, which are thought to contain RPCs. Within the stage 38 central retina, these genes are differentially expressed in the three cellular layers (GCL, INL, ONL). L, lens. (B) rx1 is expressed throughout the optic cup (arrow). (C) At stage 38, rx1 is expressed in all zones of the CMZ and weakly in the ONL (arrow). (D) pax6 is expressed throughout the optic cup (arrow). (E) At stage 38, pax6 is expressed in the CMZ and throughout the GCL and INL. (F) six3 is expressed throughout the optic cup (arrow). (G) At stage 38, six3 is expressed throughout the CMZ, and in the GCL and INL; the faint staining in the ONL is due to the thickness of the section. (H) notch1 is expressed throughout the optic vesicle (arrow). (I) At stage 38, notch1 is expressed in zones 2 and 3 of the CMZ and throughout the GCL and INL. (J) sox2 is expressed throughout the optic vesicle (arrow). (K) At stage 38, sox2 is expressed in all zones of the CMZ, strongly in the GCL and is barely detected in the INL. (L) ngn1 is expressed in the central part of the optic cup (arrow). (M) At stage 38, ngn1 is expressed in all layers of the central retina, and is absent from the CMZ. (N) p27 is expressed broadly in the optic cup (arrow). (O) At stage 38, p27 is expressed in zones 2 and 3 of the CMZ and throughout the GCL and INL. (P) neuroD is expressed broadly in the optic cup (arrow). (Q) At stage 38, neuroD is expressed in zone 3 of the CMZ and throughout the GCL and INL. (R) Xath5 is expressed broadly in the optic cup (arrow). (S) At stage 38, Xath5 is expressed in zone 3 of the CMZ and throughout the GCL and INL.

Image published in: Zaghloul NA and Moody SA (2007)

Copyright © 2007. Image reproduced with permission of the Publisher, Elsevier B. V.

GeneSynonymsSpeciesStage(s)Tissue
six3.Lhpe2, six3-a, six3-b, XSix3, xsix3.1, xsix3.2X. laevisThroughout NF stage 21eye
oral epithelium
eye primordium
notch1.Snotch, notch-1, x-notch-1, xnotch, xnotch1, xotchX. laevisThroughout NF stage 22retina
eye
neural plate
spinal cord
diencephalon
hindbrain
eye primordium
pax6.Lan2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6X. laevisThroughout NF stage 20retina
optic vesicle
lens placode
diencephalon
neural tube
eye primordium
sox2.Lanop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2X. laevisThroughout NF stage 22retina
neural plate
optic vesicle
lens placode
anterior neural tube
spinal cord
hindbrain
midbrain
eye primordium
rax.Lrax-a, rax-b, rax1, rx, rx-1, rx1, rx1a, Rx2A, Xrax, Xrx1, Xrx1AX. laevisThroughout NF stage 20retina
eye
optic vesicle
eye primordium
neurod1.Sneurod, neuroD, neurod1-a, neurod1-b, XNeuroDX. laevisThroughout NF stage 26retina
optic vesicle
epibranchial placode
atoh7.Lath5, atoh7-a, atoh7-b, math5, Xath5, Xath5bX. laevisThroughout unspecified stageretina
optic vesicle
eye primordium
neurog1.Lneurod3, neurogenin, neurogenin 1, neurogenin-1, neurogenin1, ngn, ngn1X. laevisThroughout NF stage 26retina
optic vesicle
lens placode
forebrain
anterior neural tube
hindbrain
midbrain
epibranchial placode
six3.Lhpe2, six3-a, six3-b, XSix3, xsix3.1, xsix3.2X. laevisThroughout NF stage 37 and 38retina
retinal ganglion cell layer
notch1.Snotch, notch-1, x-notch-1, xnotch, xnotch1, xotchX. laevisThroughout NF stage 37 and 38retina
eye
retinal ganglion cell layer
pax6.Lan2, mgda, pax-6, pax6-a, pax6-b, wagr, XLPAX6, xpax6X. laevisThroughout NF stage 37 and 38lens
ciliary marginal zone
retina
eye
retinal inner nuclear layer
retinal ganglion cell layer
sox2.Lanop3, LOC108718087, mcops3, Sox-2, XLSOX-2, Xsox-2, XSox2X. laevisThroughout NF stage 37 and 38lens
ciliary marginal zone
retina
neurog1.Lneurod3, neurogenin, neurogenin 1, neurogenin-1, neurogenin1, ngn, ngn1X. laevisThroughout NF stage 37 and 38lens
retina
lens placode
retinal ganglion cell layer
retinal inner nuclear layer
retinal outer nuclear layer
retinal pigmented epithelium
cdknx.Lp27Xic-1, p27XIC1, p28, Xic-1, Xic1X. laevisThroughout NF stage 27retina
eye
hindbrain
midbrain
epibranchial placode
cdknx.Lp27Xic-1, p27XIC1, p28, Xic-1, Xic1X. laevisThroughout NF stage 37 and 38lens
retina
lens placode
retinal ganglion cell layer
atoh7.Lath5, atoh7-a, atoh7-b, math5, Xath5, Xath5bX. laevisThroughout NF stage 37 and 38retina
eye
retinal ganglion cell layer

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