Fig. 6.Spatial pattern of expression of TSP-1 mRNA. Whole mount in situ hybridization, using digoxygenin-labeled antisense TSP-1 probes, was performed on albino Xenopus embryos. Experiments with TSP-1 probe in the sense orientation were regularly performed to control for specificity and these embryos lacked detectable staining (data not shown). Each embryo is oriented with anterior to the right. Embryos were staged according to Nieuwkoop and Faber (1994). Stage 19 neurula, (A) dorsal view (B) side view. Arrows indicate single line of cells along the dorsal midline. Arrowheads indicate bilaterally symmetrical staining in the hindbrain. C: Stage 26 tailbud, side view. Arrow indicates floor of forebrain. Arrowhead indicates endoderm ventrally apposed to the notohord. D: Stage 28 tailbud, side view. Arrowhead indicates epidermal staining surrounding the cement gland. E: Stage 30 tailbud, side view. Arrowhead indicates hindbrain staining in alternating rhombomeres. White arrow points to expression in the caudal somites. Black arrow indicates notochord staining. F: Stage 32 tailbud, side view. Note the increased staining within caudal somites and rhombomeres in comparison to (E). G: Stage 34 tailbud, side view. Arrow indicates intense expression in the pre-chordal plate. Arrowhead points to expression in anterior epidermis. H: Stage 40 tadpole, side view. Arrow indicates staining in the heart. Note the lack of somitic expression.
Image published in: Urry LA et al. (1998)
Copyright © 1998. Image reproduced with permission of the Publisher, John Wiley & Sons.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| thbs1.L | thbs, thbs-1, thrombospondin 1, tsp, tsp-1, tsp1 | X. laevis | Throughout NF stage 19 | floor plate neural tube neuroectoderm presumptive midbrain-hindbrain boundary |
| thbs1.L | thbs, thbs-1, thrombospondin 1, tsp, tsp-1, tsp1 | X. laevis | Throughout NF stage 26 | neural tube forebrain floor plate endoderm hypochord |
| thbs1.L | thbs, thbs-1, thrombospondin 1, tsp, tsp-1, tsp1 | X. laevis | Throughout NF stage 28 | notochord floor plate neural tube hypochord endoderm cement gland cranial ganglion |
| thbs1.L | thbs, thbs-1, thrombospondin 1, tsp, tsp-1, tsp1 | X. laevis | Sometime during NF stage 29 and 30 to NF stage 32 | mesoderm notochord neural tube hindbrain cranial ganglion cement gland tail somite rhombomere R2 rhombomere R3 rhombomere R4 rhombomere R5 trunk somite neuroectoderm |
| thbs1.L | thbs, thbs-1, thrombospondin 1, tsp, tsp-1, tsp1 | X. laevis | Throughout NF stage 33 and 34 | epidermis mesoderm notochord neural tube hindbrain rhombomere R2 rhombomere R3 rhombomere R4 rhombomere R5 cranial ganglion olfactory region otic vesicle trunk somite tail somite prechordal plate |
| thbs1.L | thbs, thbs-1, thrombospondin 1, tsp, tsp-1, tsp1 | X. laevis | Throughout NF stage 40 | epidermis heart head region tail fin |
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