Fig. 3. Expression of X. tropicalis TLEs. Expression patterns of TLE1, TLE2, AES and FoxG1 as shown by whole-mount in situ hybridisation of X. tropicalis embryos at neural plate stage (st. 16; frontal view), early tailbud (st. 22-23; frontal view), and late tailbud (st. 28; lateral view). TLE1 and TLE2 show expression throughout the anterior neural plate (st. 16), but TLE1 is strongest in the anterior neural ridge (black arrow), whereas TLE2 is strongest in a more medial ridge (black arrow). Note the expression of TLE1, but not TLE2, in the forebrain of st. 28 embryos, which largely overlaps with the telencephalic expression of FoxG1 (telencephalon indicated by red arrowheads). Ey, eye; m, midbrain; h, hindbrain; ba, brancial arches; di, diencephalon; ov, otic vesicle.