Larger Image Fig. 5. Whole-mount in situ hybridization of Xksy mRNA during Xenopus embryogenesis. Embryos were hybridized overnight to the DIG-labeled Xksy anti- sense cRNA probe, incubated with anti-digoxigenin-AP antibody, and subjected to color development (A,C,E,F,G). Xksy sense probe was included as a control for non-specific hybridization (B,D). (A) Stage 6.5 (blastula); lateral view. Asterisks, vegetal hemisphere. (B,C) Stage 11–11.5 (gastrula); lateral view. Asterisks, yolk plug. (D,E) Stage 40 (tadpole); lateral view. (F,G) Head region of stage 40; (F) lateral view, (G) dorsal view. fb, forebrain; mb, midbrain; hb, hindbrain; sc, spinal cord; np, nasal placodes; ov, optic vesicles; ls, lens; br, branchial arches. Image published in: Kishi YA et al. (2002) Copyright © 2002. Image reproduced with permission of the Publisher, Elsevier B. V.
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