FIGURE 9. GRAF1 is highly expressed during somitogenesis. A, RT-PCR analysis for x.GRAF1 and histone H4 (H4; loading control) was performed using RNA isolated from embryos at the indicated stages. B and C, whole-mount in situ hybridization of stage 29 Xenopus embryo using an antisense probe specific for x.GRAF1: h, heart; b, brain; s, somites; nt, neural tube; drg, dorsal root ganglia. B, lateral view. C, top, transverse; bottom; cross-section through mid-somite region (somite borders indicated with brackets). D, Western blot analysis of GRAF1 and ERK (loading control) expression at the indicated developmental stages. E, laser scanning confocal images of whole-mount GRAF1 (red), tropomyosin (green), and ToPro3 (blue nuclei) stained somites from wild type stage 37 embryo. IHC, immunohistochemistry.
Image published in: Doherty JT et al. (2011)
Copyright © 2011. Image reproduced with permission of the Publisher and the copyright holder. This is an Open Access article distributed under the terms of the Creative Commons Attribution License.
| Gene | Synonyms | Species | Stage(s) | Tissue |
|---|---|---|---|---|
| arhgap26.L | graf, graf1, ophn1l, ophn1l1 | X. laevis | Throughout NF stage 29 and 30 | somite lens anterior ventral blood island heart spinal ganglion neural tube pharyngeal arch central nervous system brain spinal cord eye |
| arhgap26.L | graf, graf1, ophn1l, ophn1l1 | X. laevis | Throughout NF stage 29 and 30 | somite |
| tnnt2.L | CT3, LOC446916 | X. laevis | Throughout NF stage 37 and 38 | muscle somite |
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