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XB-GRNA-29231239

gRNA Name: hand1 gRNA2
Synonyms: hand1 sgT2
Genomic Target: hand1

Sequence: 5' AGGGATCAGGCATCATGTGG 3'



Genomic Target tropicalis laevis.L
Identities 19/20 20/20
genome
gRNA
5' CCACATGATGCCAGATCCCT 3'
   |||||||||||| |||||||
3' GGTGTACTACGGACTAGGGA 5'
5' CCACATGATGCCTGATCCCT 3'
   ||||||||||||||||||||
3' GGTGTACTACGGACTAGGGA 5'
    
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 10.1 Chr3L:28327970..28327989 20/20 Antisense target hand1.L
Chr1S:101842392..101842411 17/20 Sense off-target insr.S
Chr4L:92772384..92772403 17/20 Sense off-target ift25.L
Chr5L:125635284..125635303 17/20 Sense off-target mbnl1.L
Chr4S:119582461..119582480 16/20 unknown
tropicalis 10.0 Chr3:19629435..19629454 19/20 Antisense target hand1
Chr1:99490220..99490239 18/20 Sense off-target dazap1
Publications
???morpholino.displaySummary.papersFirst??? Differential requirement of Hand1 during differentiation of lateral plate mesoderm lineages in Xenopus laevis., Dev Biol 2025  
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