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XB-GRNA-29212833
gRNA Name:
csf3 gRNA1
Synonyms:
Genomic Target: csf3
PAM Sequence: TGG
Sequence: 5' GTCCTCTCAGCTCCCCTCCC 3'
Synonyms:
Genomic Target: csf3
PAM Sequence: TGG
Sequence: 5' GTCCTCTCAGCTCCCCTCCC 3'
Genomic Target | tropicalis | laevis.L | |||||
Identities | 19/20 | 20/20 | |||||
genome
gRNA
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5' GGGAGGGGCGCTGAGAGGAC 3'
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3' CCCTCCCCTCGACTCTCCTG 5' |
5' GGGAGGGGAGCTGAGAGGAC 3' |||||||||||||||||||| 3' CCCTCCCCTCGACTCTCCTG 5' |
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Genomic Alignments | Position | Identity | Strand | Target | Genomic Target | ||
laevis 10.1 | Chr9_10L:2029381..2029400 | 20/20 | Antisense | target | csf3.L | ||
Chr2L:78721445..78721464 | 18/20 | Antisense | off-target | kcnd3.L | |||
Chr9_10S:4817901..4817920 | 18/20 | Sense | off-target | rundc3a.S | |||
Chr4S:55860868..55860887 | 17/20 | Antisense | off-target | hydin.S | |||
Chr5S:138340554..138340573 | 17/20 | Sense | off-target | extl3.S | |||
Chr7S:52493927..52493946 | 17/20 | Sense | off-target | casz1.S | |||
Chr4L:145872912..145872931 | 17/20 | Antisense | off-target | adamts9.L | |||
Chr9_10L:133947911..133947930 | 17/20 | Antisense | off-target | LOC121398010 | |||
Chr1L:223817691..223817710 | 17/20 | Antisense | off-target | LOC121395843 | |||
Chr2L:163293245..163293264 | 17/20 | Sense | off-target | LOC108708695 | |||
Chr2L:163294331..163294350 | 17/20 | Sense | off-target | LOC108708695 | |||
Chr3L:112328803..112328822 | 16/20 | Sense | off-target | stard5.L | |||
Chr9_10S:12354389..12354408 | 19/20 | Sense | unknown | ||||
Chr9_10L:13657450..13657469 | 19/20 | Sense | unknown | ||||
Chr8L:7982679..7982698 | 18/20 | Antisense | unknown | ||||
Chr8L:7982708..7982727 | 18/20 | Antisense | unknown | ||||
Chr9_10L:133960416..133960435 | 17/20 | Antisense | unknown | ||||
Chr9_10L:133973556..133973575 | 17/20 | Antisense | unknown | ||||
Chr6L:152101455..152101474 | 17/20 | Antisense | unknown | ||||
Chr7S:100898838..100898857 | 17/20 | Sense | unknown | ||||
Chr9_10L:119265411..119265430 | 17/20 | Sense | unknown | ||||
Chr9_10L:133936237..133936256 | 17/20 | Antisense | unknown | ||||
Chr9_10L:133941094..133941113 | 17/20 | Antisense | unknown | ||||
Chr2S:115508365..115508384 | 16/20 | Sense | unknown | tropicalis 10.0 | Chr10:9354356..9354375 | 19/20 | Antisense | target | csf3 |
Chr1:112309798..112309817 | 19/20 | Antisense | off-target | c19orf44 | |||
Chr9:68981605..68981624 | 18/20 | Antisense | off-target | nostrin | |||
Chr4:98269850..98269869 | 18/20 | Sense | off-target | kiaa0040 | |||
Chr3:142273928..142273947 | 18/20 | Sense | off-target | LOC100491968 | |||
Chr6:2521007..2521026 | 18/20 | Sense | off-target | LOC116411724 | |||
Chr8:9617214..9617233 | 18/20 | Antisense | off-target | LOC100488020 | |||
Chr3:9557815..9557834 | 17/20 | Antisense | off-target | erc1 | |||
Chr7:16198360..16198379 | 17/20 | Antisense | off-target | fam13c | |||
Chr3:68241758..68241777 | 16/20 | Antisense | off-target | met | |||
Chr1:79259219..79259238 | 16/20 | Antisense | off-target | pkd2 | |||
Chr7:114240157..114240176 | 18/20 | Sense | unknown | ||||
Chr3:13533774..13533793 | 17/20 | Antisense | unknown | ||||
Chr7:114240312..114240331 | 17/20 | Sense | unknown | ||||
Chr7:114240467..114240486 | 17/20 | Sense | unknown | ||||
Chr7:114240549..114240568 | 17/20 | Sense | unknown | ||||
Chr7:114240631..114240650 | 17/20 | Sense | unknown | ||||
Publications | |||||||
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