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Summary Attributions Wiki
XB-GRNA-29209413
gRNA Name: myod1 gRNA1
Synonyms:
Genomic Target: myod1

Sequence: 5' ATCAGGTGCCCTGAGAGGGTGA GAGCTGCCAAGAGTCCAGATTTCCTACAACGGGGCAAGGTGACTGTGCGTGGGCATTCGGACTGGACCCCAGAGCTGTGGATCTTTTAAA CCTTCGCTGGGAGTAGTGACTTTGCGCTGTTTCT 3'



Genomic Target tropicalis laevis.S
Identities 20/148 22/148
genome
gRNA
 
5' TTACCCTCCCAGGGCACCTGAT 3'
   | |||||| |||||||||||||
3' AGTGGGAGAGTCCCGTGGACTA 5'
 
5' TCACCCTCTCAGGGCACCTGAT 3'
   ||||||||||||||||||||||
3' AGTGGGAGAGTCCCGTGGACTA 5'
     
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 10.1 Chr4S:23267685..23267706 22/22 Antisense target myod1.S
Chr1S:143024173..143024194 18/22 Sense off-target ufd1.S
Chr3S:3311405..3311426 19/22 Antisense unknown
Chr2L:106089048..106089069 18/22 Sense unknown
tropicalis 10.0 Chr4:902602..902623 20/22 Sense target myod1
Chr3:117750785..117750806 20/22 Sense off-target itga11
Chr5:68033184..68033205 19/22 Sense unknown
Publications
???morpholino.displaySummary.papersFirst??? A CRISPR-Cas9-mediated versatile method for targeted integration of a fluorescent protein gene to visualize endogenous gene expression in Xenopus laevis., Dev Biol 2024     
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