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Summary Attributions Wiki
XB-GRNA-29209273

gRNA Name: chst6 gRNA1
Synonyms: chst5.1 gRNA1
Genomic Target: chst6

PAM Sequence: NGG
Sequence: 5' CCTGATGGAACCAGCATGGCATG 3'



Genomic Target tropicalis laevis.L laevis.S
Identities 23/23 22/23 20/23
genome
gRNA
5' CATGCCATGCTGGTTCCATCAGG 3'
   |||||||||||||||||||||||
3' GTACGGTACGACCAAGGTAGTCC 5'
5' CATGCCATGCTGGTTCCATCAAG 3'
   ||||||||||||||||||||| |
3' GTACGGTACGACCAAGGTAGTCC 5'
5' CATGCCACGCTGGTTCCATCAAA 3'
   ||||||| |||||||||||||  
3' GTACGGTACGACCAAGGTAGTCC 5'
   
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 10.1 Chr4L:58234878..58234900 22/23 Antisense target chst6.L
Chr4S:45894231..45894253 20/23 Antisense target chst6.S
Chr5S:88548457..88548479 20/23 Sense off-target LOC108718044
Chr6S:87721266..87721288 19/23 Sense off-target dok6.S
Chr6S:113144446..113144468 19/23 Antisense unknown
Chr6S:64546738..64546760 19/23 Sense unknown
tropicalis 10.0 Chr4:64191079..64191101 23/23 Antisense target chst6
Chr8:1471639..1471661 20/23 Antisense off-target syn1
Chr3:38189015..38189037 19/23 Sense off-target arhgap26
Chr4:32850393..32850415 19/23 Sense off-target LOC100491113
Chr8:42513759..42513781 19/23 Antisense unknown
Publications
???morpholino.displaySummary.papersFirst??? Tissue-specific expression of carbohydrate sulfotransferases drives keratan sulfate biosynthesis in the notochord and otic vesicles of Xenopus embryos., Front Cell Dev Biol 2023     
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