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XB-GRNA-23659706
gRNA Name:
hspa5 gRNA1
Synonyms: grp78
Genomic Target: hspa5
PAM Sequence: agg
Sequence: 5' GGCAGACACCAGCAACACCA 3'
Synonyms: grp78
Genomic Target: hspa5
PAM Sequence: agg
Sequence: 5' GGCAGACACCAGCAACACCA 3'
Genomic Target | tropicalis | laevis.L | laevis.S | ||||
Identities | 20/20 | 19/20 | 20/20 | ||||
genome
gRNA
|
5' TGGTGTTGCTGGTGTCTGCC 3' |||||||||||||||||||| 3' ACCACAACGACCACAGACGG 5' |
5' TGGTGTTGATGGTGTCTGCC 3'
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3' ACCACAACGACCACAGACGG 5' |
5' TGGTGTTGCTGGTGTCTGCC 3' |||||||||||||||||||| 3' ACCACAACGACCACAGACGG 5' |
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Genomic Alignments | Position | Identity | Strand | Target | Genomic Target | ||
laevis 9.2 | chr8S:39914141..39914160 | 20/20 | Antisense | target | hspa5.S | ||
chr8L:10666541..10666560 | 19/20 | Antisense | target | hspa5.L | |||
chr3S:104077903..104077922 | 19/20 | Antisense | off-target | adrb2.S | |||
chr5L:79057610..79057629 | 18/20 | Sense | off-target | ak9.L | |||
chr5L:79057610..79057629 | 18/20 | Sense | off-target | LOC108716836 [provisional:ak9] | |||
chr5S:62549244..62549263 | 17/20 | Antisense | off-target | bves.S | |||
chr7L:84260614..84260633 | 17/20 | Sense | off-target | pex10.L | |||
Scaffold221:19242..19261 | 18/20 | Sense | unknown | ||||
Scaffold55:416670..416689 | 18/20 | Antisense | unknown | ||||
chr5L:8560839..8560858 | 18/20 | Sense | unknown | ||||
chr6L:27899315..27899334 | 18/20 | Antisense | unknown | ||||
chr2L:113032054..113032073 | 17/20 | Antisense | unknown | ||||
chr4S:38629592..38629611 | 17/20 | Antisense | unknown | ||||
chr8L:88529453..88529472 | 17/20 | Antisense | unknown | ||||
chr9_10L:56527552..56527571 | 16/20 | Antisense | unknown | tropicalis 10.0 | Chr8:28185626..28185645 | 20/20 | Antisense | target | hspa5 |
Chr9:31011075..31011094 | 17/20 | Antisense | off-target | mad1l1 | |||
Chr1:16365533..16365552 | 16/20 | Sense | off-target | LOC101734035 | |||
Publications | |||||||
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