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XB-GRNA-23659494
gRNA Name:
zbtb21 gRNA2
Synonyms: trop zbtb21 T7
Genomic Target: zbtb21
PAM Sequence: AGG
Sequence: 5' TGTTTGTCAGAACCGAATTGAGG 3'
Synonyms: trop zbtb21 T7
Genomic Target: zbtb21
PAM Sequence: AGG
Sequence: 5' TGTTTGTCAGAACCGAATTGAGG 3'
Genomic Target | tropicalis | laevis.L | laevis.S | ||||
Identities | 23/23 | 22/23 | 21/23 | ||||
genome
gRNA
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5' CCTCAATTCGGTTCTGACAAACA 3' ||||||||||||||||||||||| 3' GGAGTTAAGCCAAGACTGTTTGT 5' |
5' CCTCAAGTCGGTTCTGACAAACA 3'
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3' GGAGTTAAGCCAAGACTGTTTGT 5' |
5' CCTCAAGTCGGCTCTGACAAACA 3' |||||| |||| ||||||||||| 3' GGAGTTAAGCCAAGACTGTTTGT 5' |
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Genomic Alignments | Position | Identity | Strand | Target | Genomic Target | ||
laevis 9.2 | chr2L:9011557..9011579 | 22/23 | Sense | target | zbtb21.L | ||
chr2S:23186972..23186994 | 21/23 | Antisense | target | zbtb21.S | |||
chr1S:53339421..53339443 | 19/23 | Sense | off-target | fat4.S | |||
chr8S:22091717..22091739 | 19/23 | Sense | off-target | ptpn21.S | |||
chr5L:36239519..36239541 | 21/23 | Sense | unknown | ||||
chr5S:54431575..54431597 | 19/23 | Sense | unknown | tropicalis 10.0 | Chr2:9601056..9601078 | 23/23 | Sense | target | zbtb21 |
Chr1:120059582..120059604 | 20/23 | Sense | off-target | abca1 | |||
Chr4:74984420..74984442 | 19/23 | Antisense | off-target | fcsk | |||
Publications | |||||||
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