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Summary Attributions Wiki
XB-GRNA-23659460
gRNA Name: sf3b4 gRNA1
Synonyms: trop sf3b4 T5
Genomic Target: sf3b4

PAM Sequence: GGG
Sequence: 5' GCGGCGAGAAAATGGCGGCTGGG 3'



Genomic Target tropicalis laevis.L laevis.S
Identities 23/23 21/23 22/23
genome
gRNA
 
5' CCCAGCCGCCATTTTCTCGCCGC 3'
   |||||||||||||||||||||||
3' GGGTCGGCGGTAAAAGAGCGGCG 5'
 
5' ACCGGCCGCCATTTTCTCGCCGC 3'
    || |||||||||||||||||||
3' GGGTCGGCGGTAAAAGAGCGGCG 5'
 
5' CCCAGCCGCCATTTTCACGCCGC 3'
   |||||||||||||||| ||||||
3' GGGTCGGCGGTAAAAGAGCGGCG 5'
    
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 9.2 chr8S:91668541..91668563 22/23 Antisense target sf3b4.S
chr8L:105108248..105108270 21/23 Antisense target sf3b4.L
chr6L:138870399..138870421 20/23 Sense off-target ext1.L
tropicalis 10.0 Chr8:128890380..128890402 23/23 Antisense target sf3b4
Chr2:29254719..29254741 19/23 Antisense off-target glra2
Chr2:115370368..115370390 21/23 Antisense unknown
Publications
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