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XB-GRNA-23659447
gRNA Name:
rfx6 gRNA1
Synonyms: trop rfx6T
Genomic Target: rfx6
PAM Sequence: AGG
Sequence: 5' TGGGCATACAGTATGCATCGAGG 3'
Synonyms: trop rfx6T
Genomic Target: rfx6
PAM Sequence: AGG
Sequence: 5' TGGGCATACAGTATGCATCGAGG 3'
Genomic Target | tropicalis | laevis.L | |||||
Identities | 23/23 | 21/23 | |||||
genome
gRNA
|
5' CCTCGATGCATACTGTATGCCCA 3' ||||||||||||||||||||||| 3' GGAGCTACGTATGACATACGGGT 5' |
5' CCTCGATGCATACTCTATGCTCA 3' |||||||||||||| ||||| || 3' GGAGCTACGTATGACATACGGGT 5' |
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Genomic Alignments | Position | Identity | Strand | Target | Genomic Target | ||
laevis 9.2 | chr5L:75534039..75534061 | 21/23 | Sense | target | rfx6.L | ||
chr8L:84750235..84750257 | 21/23 | Sense | off-target | fntb.L | |||
chr8L:84750235..84750257 | 21/23 | Sense | off-target | slc8a3.L | |||
chr4L:26012303..26012325 | 20/23 | Sense | off-target | atl3.L | |||
chr9_10L:89263409..89263431 | 19/23 | Antisense | off-target | zc3h7a.L | |||
chr1L:139641984..139642006 | 19/23 | Sense | off-target | LOC101027262 | |||
chr5S:57009809..57009831 | 21/23 | Sense | unknown | ||||
chr1L:191967751..191967773 | 20/23 | Sense | unknown | ||||
chr1S:59324612..59324634 | 19/23 | Antisense | unknown | ||||
chr3L:16248133..16248155 | 19/23 | Antisense | unknown | ||||
chr5L:107327380..107327402 | 19/23 | Sense | unknown | tropicalis 10.0 | Chr5:74668842..74668864 | 23/23 | Sense | target | rfx6 |
Chr1:125419237..125419259 | 19/23 | Sense | unknown | ||||
Chr1:71413412..71413434 | 19/23 | Antisense | unknown | ||||
Chr3:93612194..93612216 | 19/23 | Antisense | unknown | ||||
Chr5:138353635..138353657 | 19/23 | Antisense | unknown | ||||
Publications | |||||||
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