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Summary Attributions Wiki
XB-GRNA-23659434
gRNA Name: pnhd gRNA1
Synonyms: pinhead, trop pnhd T4
Genomic Target: pnhd

PAM Sequence: AGG
Sequence: 5' GAAGAATGCTTTGCAAGTGCAGG 3'



Genomic Target tropicalis laevis.L laevis.S
Identities 23/23 21/23 22/23
genome
gRNA
 
5' CCTGCACTTGCAAAGCATTCTTC 3'
   |||||||||||||||||||||||
3' GGACGTGAACGTTTCGTAAGAAG 5'
 
5' CCTGGGCTTGCAAAGCATTCTTC 3'
   ||||  |||||||||||||||||
3' GGACGTGAACGTTTCGTAAGAAG 5'
 
5' CCTGCACTTGCAAAGCATTCGTC 3'
   |||||||||||||||||||| ||
3' GGACGTGAACGTTTCGTAAGAAG 5'
    
Genomic Alignments Position Identity Strand Target Genomic Target
laevis 9.2 chr1S:89991173..89991195 22/23 Antisense target pnhd.S
chr1L:101959506..101959528 21/23 Antisense target pnhd.L
chr6L:57147887..57147909 20/23 Antisense off-target pkd1l1.L
chr7L:42524547..42524569 19/23 Sense off-target unc5b.L
chr2S:4982877..4982899 20/23 Antisense unknown
chr8S:79397397..79397419 20/23 Antisense unknown
Scaffold71230:141..163 19/23 Antisense unknown
chr1S:179940182..179940204 19/23 Antisense unknown
chr8L:40392316..40392338 19/23 Sense unknown
tropicalis 10.0 Chr1:104460364..104460386 23/23 Antisense target pnhd
Chr3:110766750..110766772 19/23 Sense off-target ntrk3
Chr4:77098959..77098981 19/23 Sense off-target ddah1
Chr4:92396082..92396104 19/23 Antisense off-target mast2
Chr6:134334583..134334605 19/23 Antisense off-target ebag9
Chr10:8176595..8176617 19/23 Antisense unknown
Chr4:77201572..77201594 19/23 Sense unknown
Publications
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