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XB-GRNA-23659410
gRNA Name:
mtnr1a gRNA4
Synonyms: trop mtnr1a T2
Genomic Target: mtnr1a
PAM Sequence: TGG
Sequence: 5' AGACTGAGAGGATAACCAGGTGG 3'
Synonyms: trop mtnr1a T2
Genomic Target: mtnr1a
PAM Sequence: TGG
Sequence: 5' AGACTGAGAGGATAACCAGGTGG 3'
| Genomic Target | tropicalis | laevis.L | laevis.S | ||||
| Identities | 22/23 | ||||||
genome
gRNA
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5' CCTCCTGGTTATCCTCTCAGTCT 3' || |||||||||||||||||||| 3' GGTGGACCAATAGGAGAGTCAGA 5' | ||||||
Genomic Alignments  |
Position | Identity | Strand | Genomic Target | |||
| laevis 9.2 | chr9_10L:62519865..62519887 | 19/23 | Sense | off-target | LOC108701348 | ||
| chr9_10S:57342319..57342341 | 19/23 | unknown | tropicalis 10.0 | Chr1:43686128..43686150 | 22/23 | Antisense | mtnr1a |
| Chr1:104187612..104187634 | 20/23 | Antisense | il12rb1 | ||||
| Chr3:42112796..42112818 | 19/23 | ||||||
| Chr9:71162728..71162750 | 19/23 | ||||||
| Publications | |||||||
| First: |
CRISPR/Cas9 mediated mutation of the mtnr1a melatonin receptor gene causes rod photoreceptor degeneration in developing Xenopus tropicalis., Sci Rep 2020  |
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| Most recent: | |||||||
| View All Papers | |||||||
