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XB-GRNA-23659403
gRNA Name:
mapk10 gRNA1
Synonyms: trop mapk10 T1
Genomic Target: mapk10
PAM Sequence: TGG
Sequence: 5' AATCTAAAACCTATAGGCTCTGG 3'
Synonyms: trop mapk10 T1
Genomic Target: mapk10
PAM Sequence: TGG
Sequence: 5' AATCTAAAACCTATAGGCTCTGG 3'
Genomic Target | tropicalis | laevis.L | laevis.S | ||||
Identities | 23/23 | 21/23 | 21/23 | ||||
genome
gRNA
|
5' CCAGAGCCTATAGGTTTTAGATT 3' ||||||||||||||||||||||| 3' GGTCTCGGATATCCAAAATCTAA 5' |
5' CCAGAACCTATAGGTTTCAGATT 3' ||||| ||||||||||| ||||| 3' GGTCTCGGATATCCAAAATCTAA 5' |
5' CCAGACCCTATAGGTTTCAGATT 3' ||||| ||||||||||| ||||| 3' GGTCTCGGATATCCAAAATCTAA 5' |
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Genomic Alignments | Position | Identity | Strand | Target | Genomic Target | ||
laevis 9.2 | Scaffold26:2437117..2437139 | 21/23 | Antisense | target | mapk10.S | ||
chr1L:80660153..80660175 | 21/23 | Sense | target | mapk10.L | |||
chr5L:99972188..99972210 | 20/23 | Sense | off-target | tprg1.L | |||
chr7S:28186671..28186693 | 19/23 | Antisense | off-target | mcu.S | |||
chr9_10L:100160130..100160152 | 19/23 | Antisense | off-target | ocm1.L | |||
chr2L:68574449..68574471 | 19/23 | Sense | off-target | c1orf116.L | |||
chr9_10L:41966198..41966220 | 19/23 | Antisense | off-target | LOC108701185 [provisional:myh10] | |||
chr4S:69607874..69607896 | 19/23 | Sense | unknown | tropicalis 10.0 | Chr1:87073703..87073725 | 23/23 | Antisense | target | mapk10 |
Publications | |||||||
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